雾化吸入灭活草分支杆菌减轻哮喘小鼠气道炎症及对Toll样受体2表达的影响  

Effects of inhaled inactivated-Mycobacterium phlei on airway inflammation and expression of TLR2 in asthmatic mice

作　　者：张景鸿[1] 李超乾[1] 郭素娟[1] 李意[1] 严乐涛[1] 

机构地区：[1]广西医科大学第一附属医院呼吸内科,南宁530021

出　　处：《免疫学杂志》2012年第1期19-23,共5页Immunological Journal

摘　　要：目的研究雾化吸入灭活草分支杆菌对支气管哮喘小鼠气道炎症及肺组织细胞因子分泌的影响,探讨Toll样受体2(TLR2)表达在雾化吸入灭活草分支杆菌防治支气管哮喘中的作用。方法将24只雄性Balb/c小鼠按随机数字表法分为3组,每组8只:正常对照组(A)、哮喘模型组(B)、干预组(C)。卵清蛋白(OVA)致敏制小鼠支气管哮喘模型。C组在每次卵蛋白激发前给予雾化吸入草分枝杆菌治疗,每天1次。各组动物处死后提取肺组织和支气管肺泡灌洗液(BALF)。进行病理HE染色、AB-PAS染色观察支气管肺炎症和气道粘液分泌情况,并行半定量分析。BALF中炎症细胞计数,检测BALF中IL-4、IL-10、IFN-γ水平。实时定量PCR检测肺组织TLR2 mRNA表达水平。结果干预组BALF中IL-4分泌减少,IL-10、IFN-γ增加(P<0.05),BALF中嗜酸性粒细胞比例低于模型组,气道炎症病变较模型组减轻,肺组织TLR2 mRNA表达水平较模型组显著升高(P<0.05)。结论吸入草分枝杆菌能减轻支气管哮喘小鼠气道炎症,其效应与调节肺内细胞因子分泌有关。草分枝杆菌可能通过上调TLR2基因的表达调节支气管哮喘的免疫失衡。Studies have shown Mycobacterium infections suppress airway hyperresponsiveness and bovis Bacille Calmette-Gu6rin （BCG） and other mycobacterial inflammation in asthma. We use a murine model of asthma to study whether nebulized inhalation of inactivated Mycobacterium phlei can alleviate asthmatic airway inflammation through influencing cytokine production and TLR2. Male Balb/c mice were made a murine asthma model with OVA. Lung tissue and bronchoalveolar lavage fluid （BALF） were harvested. Mycobacterium phlei treat- ment decreased IL-4 production but increased IL-10 or IFN-γ in BALF（P〈0.05）; the levels of TLR2 mRNA were increased after Mycobacterium phlei treatment. Inflammatory cells counting and histological examination of the lungs showed airway inflammation were attenuated because of using Mycobacterium phlei. In conclusion, the inhalation of Mycobacterium phlei can reduce airway inflammation in asthmatic mice, and this ability was associated with its immunomodulatory effect on expression. regulating IL-4, IL-10 and IFN-γ secretion and TLR2

关 键 词：支气管哮喘 草分枝杆菌 黏膜免疫 TLR2 

分 类 号：R562.25[医药卫生—呼吸系统]