西伯利亚百合不同部位鳞片的快速繁育  

Rapid Propagation of Scales in Different Parts of Lilium×siberia

作　　者：王淑芳[1] 李凤华[1] 张仁波[1] 

机构地区：[1]遵义师范学院生物系,贵州遵义563002

出　　处：《贵州农业科学》2011年第12期40-42,共3页Guizhou Agricultural Sciences

基　　金：遵义市科技局资助项目"根癌农杆菌介导的CryIA抗虫基因转化生姜的研究"[2010(16)]

摘　　要：为西伯利亚百合种质保存、工厂化育苗及遗传改良提供依据,以西伯利亚百合的外、中、内3层鳞片为外植体进行组织培养研究,建立了西伯利亚百合鳞片的快速繁殖体系。结果表明:中部鳞片的小磷茎诱导率最高,MS+6-BA 2.0mg/L+NAA 0.2mg/L为小鳞茎最佳诱导培养基,诱导率达85.7%;试管苗生根培养基以1/2MS+IAA 0.2mg/L最好,生根率达96.7%。An efficient in vitro plant regeneration system and clonal propagation via bulb scales of Lilium×siberia was established to provide references for germplasm preservation,industrial seed culture and genetic improvement of Lilium×siberia.The results showed that the best bulblets induction（85.7%） was achieved from bulb scales cultured on MS medium containing 2.0 mg/L 6-BA and 0.2 mg/L NAA,The developed bulblets were rooted on 1/2 MS medium supplemented with 0.2 mg/L IAA,the root regeneration ratio was 96.7%.

关 键 词：西伯利亚百合 快速繁殖 鳞片 

分 类 号：S682.2[农业科学—观赏园艺]