免疫法和化学法检测粪隐血的方法评价  

Immunoassay and Chemical Method of Evaluation of Fecal Occult Blood Test

作　　者：梁巍 潘莉 王域平 

机构地区：[1]信阳市中心医院检验科,河南信阳464000

出　　处：《中国医药指南》2011年第36期19-20,共2页Guide of China Medicine

摘　　要：目的评价免疫胶体金法和化学法检测粪便隐血结果,探讨这二种检测方法的优劣和应用。方法用邻联甲苯胺和便隐血免疫胶体金检测试纸分别检测260例粪标本进行对照实验并分析。结果化学法阳性率为10.6%,免疫胶体金法法阳性率为19.4%。结论化学法灵敏度、特异性较胶体金法低,受饮食影响因素大,胶体金法有假阴性,故在临床工作中两种方法可结合使用,以便获得更准确的实验结果。Objective We assess the result of both immune colloidal gold assay and chemical assay to test fecal occult blood and compared the two methods in this paper. Methods We use o-tolidine and immune colloidal gold test paper to test 260 excrement samples respectively, then compare and analysis. Results We find 10.6% is positive by chemical assay while 19.4% is positive by immune colloidal gold assay. Conclusions So we can tell that the chemical assay has high sensitivity yet low specificity, which is affected by diet easily. However, immune colloidal gold assay might turn out to be false negative error. In conclusion, we can combine the two assays clinically to get the experimental result precisely.

关 键 词：大便隐血 邻联甲苯胺法 免疫胶体金 

分 类 号：R446.133[医药卫生—诊断学]