维吾尔族慢性HBV感染者HBV RT区准种研究  

Characteristics of HBVquasispecies in Uygur patients with chronic hepatitis B virus infection in Xinjiang

作　　者：鲁晓擘[1] 王晓岚[1] 沙尼亚.尼亚孜 汤影子[2] 刘霖[2] 张跃新[1] 王宇明[2] 

机构地区：[1]新疆医科大学第一附属医院感染科,乌鲁木齐830011 [2]第三军医大学西南医院感染科,重庆400038

出　　处：《新疆医科大学学报》2011年第9期1010-1015,共6页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区重点学科资助项目(2004-2008);新疆医科大学第一附属医院科研奖励基金(2008-YFY02)

摘　　要：目的初步探讨新疆维吾尔族慢性HBV感染者HBV准种特性。方法选择5例维吾尔族慢性HBV感染者(抗乙型肝炎病毒治疗与未治疗)为主要研究对象,选择HBV RT区为研究靶区。采用克隆-测序方法进行准种研究。使用BioEdit和NCBI-BLAST工具进行生物信息学分析,并探讨其与临床的关系。结果治疗的患者(拉米夫定)HBV准种复杂性为12、14,未治疗者HBV准种复杂性为7、8、10、5。核酸变异的主要形式为点突变,包括碱基的替换和缺失,所有标本的优势克隆在HBV RT区"热点"变异位点V173、L180、A181、A184、S202、M204、N236和M250位均未发生变异。治疗和未治疗患者HBV的优势克隆均发生了rtA222T(A:丙氨酸;T:苏氨酸)突变。1例患者HBV准种动力学结果提示,病情重时其准种复杂性为8,好转时复杂性为10。结论治疗患者(拉米夫定)HBV准种复杂性似要高于未治疗者,未经药物选择下HBV准种组成相对较单一。HBV准种群会发生漂变,即病情重时其准种复杂性低,病情好转时复杂性上升。Objective To study characteristic of hepatitis B virus（HBV） quasispecies in Uygur patients with chronic hepatitis B infection in Xinjiang.Methods We choose five Uygur patients with chronic HBV infection,including chronic hepatitis B and liver cirrhosis,and one of those is acute exacerbation on chronic hepatitis B.We determine quasispecies of HBV reverse transcriptase（HBV RT） region by using PCR-Clone-sequence analysis.HBV RT region was amplified and cloned by nested PCR and TA cloning.33 clones from a single sample were measured by sequence analysis to determine the different clonetypes.Results The complexity and heterogeneity of quasispecies of the region were 12 and 14 with lamivudine treatment,and were 7,8,10 and 5 without lamivudine treatment.The complexity and heterogeneity of quasispecies of this region were low（8） at the time points of exacerbation of chronic hepatitis and high（10） at the time points of recovery of chronic hepatitis.The main style of variant is point mutation.V173,L180,A181,A184,S202,M204,N236,M250 mutations have not been detected in RT gene in the predominant clones of HBV strain.rtA222T mutation has been detected in RT gene in the predominant clones of HBV strain that can not be detected usually.Conclusion There exist quasispecies phenomena in HBV RT region in Uygur CHB patients in Xinjiang.It is possible that the shift of quasispecies of HBV RT region is related with antiviral treatment and clinical courses.The complexity and heterogeneity of quasispecies of this region were low at the time points of exacerbation of chronic hepatitis and high at the time points of recovery of chronic hepatitis,and were higher with lamivudine treatment than no treatment.The complexity and heterogeneity of quasispecies of RT region may be unity without drug pressure.

关 键 词：维吾尔族 乙型肝炎病毒 逆转录酶区 准种 

分 类 号：R34[医药卫生—基础医学] R512.6