反相高效液相色谱法测定昆仑雪菊中绿原酸的含量  

Determination of chlorogenic acid in kunlun chrysanthemum by reversed phase high performance liquid chromatograpy

作　　者：买买提.艾买提 赵文惠[2] 木合布力.阿布力孜 李新霞[2] 

机构地区：[1]新疆哈密地区药品检验所,新疆哈密839000 [2]新疆医科大学药学院,乌鲁木齐830011

出　　处：《新疆医科大学学报》2011年第12期1370-1372,共3页Journal of Xinjiang Medical University

摘　　要：目的建立昆仑雪菊中绿原酸的含量测定方法。方法采用反相高效液相色谱法,色谱柱:WatersXTerra RP18(150mm×4.6 mm,3.5μm),流动相:乙腈-0.1%磷酸(15∶85),检测波长:326nm,流速:1.0mL/min。结果绿原酸在8.05～120.72μg/mL范围内线性关系良好,平均回收率为101.0%,RSD为3.1%。结论反相高效液相色谱法简便、准确,专属性强,可以用于昆仑雪菊中绿原酸的含量测定。Objective the content determination method of chlorogenic acid Kunlun chrysanthemum. Methods Using High Performance Liquid Chromatography Column： in Waters XTerra RP18（150 mm ×4. 6 mm, 3.5μm）, Mobile phase：Acetonitrile-0.1%phosphoric acid （15：85）, Detection wavelength： 326nm, Flow rate： 1.0 ml/min. ResultsChlorogenicacid was ina goodlinear relationshipwith the concentration of 8.05-120.72μg/ml. The average recovery was 101.0%, RSD was 3.1%. Conclusion Thismethod is a convenient,accurate, specific andcan be usedin content determination of chlorogenic acid inKunlun chrysanthemum.To establish

关 键 词：反相高效液相色谱 昆仑雪菊花 绿原酸 

分 类 号：R927.2[医药卫生—药学] R284.1