CIK细胞的体外扩增能力及杀瘤效应  

Study of the expansion in vitro and its cytotoxicity on CIK cell

作　　者：郭云泉[1] 赵峰[1] 梁丽萍[1] 张亚楼[2] 

机构地区：[1]新疆医科大学附属肿瘤医院病理科,乌鲁木齐830011 [2]新疆医科大学基础医学院组织胚胎学教研室,乌鲁木齐830011

出　　处：《新疆医科大学学报》2011年第11期1244-1246,共3页Journal of Xinjiang Medical University

基　　金：新疆医科大学科研创新基金资助(2008-3-1)

摘　　要：目的研究CIK细胞的体外生长扩增能力及杀瘤效应。方法将人外周血用淋巴细胞分离液分离出外周血单核细胞(PBMC),加入不同的细胞因子(IFN-γ、CD3mAb、IL-1、IL-2),诱导生成CIK细胞,分为CIK-1组和CIK-2组,观察CIK细胞的扩增情况,用流式细胞仪检测其表面标志,MTT法测定其杀瘤活性。结果 CIK细胞中CD3+CD56+细胞经过21d培养后显著增加,百分比达(29.80±8.73)%,与培养前相比差异有统计学意义(P<0.05),CD8+细胞也较培养前显著增加(P<0.05)。CIK细胞具有较强的杀瘤能力,在第7d时CIK-1组杀瘤活性为57.86%,第14d时为71.92%,在第21d时达最高值,为79.06%,与CIK-2组比较杀伤活性显著增高,差异有统计学意义(P<0.05)。结论 CIK细胞具有较强的体外扩增能力和杀瘤效应,为肿瘤临床治疗提供了一种新抗肿瘤和主动免疫治疗方法。Objective To research the proliferation ability in vitro and cytotoxicity of CIK cells.Methods Human peripheral blood mononuclear cells（PBMC） were isolated with ficoll solution.CIK cells from PBMC were induced with different cytokines（IFN-γ,CD3mAb,IL-1,IL-2）,the proliferation ability of CIK cells was measured and calculated,the phenotype of CIK cells was analyzed by flow cytometer and the killing cancer cell activity of CIK cells was analyzed with MTT test.Results CIK cells belong to a heterogeneous group of effect cells and possess very high proliferate ability,CD3＋CD56＋ cells was the major effect cell of CIK cell group.The number of CD3＋CD56＋ cells had a significant increase in the percentage rate.The percentage was to（29.80±8.73）% after 21 days culturing.CIK cell had significantly higher cytotoxicity.CIK-1 group got 57.86% of cytotoxicity after 7 days culturing,71.92% of cytotoxicity after 14days culturing and hit the peak of 79.06% after 21 days culturing.Conclusion CIK cells had a high proliferation ability and cytotoxicity.It might be used as a new anti-tumor or adoptive immunotherapy in clinic.

关 键 词：CIK细胞 扩增 杀瘤效应 

分 类 号：R392.12[医药卫生—免疫学]