小麦花培苗染色体加倍技术研究  

Chromosome Doubling Technique for Anther Culture of Winter Wheat

作　　者：杨丽萍[1] 朱晋云[1] 李楠[1] 

机构地区：[1]山西省农业科学院小麦研究所,山西临汾041000

出　　处：《生物技术进展》2011年第6期436-439,共4页Current Biotechnology

基　　金：山西省农业科学院高新技术项目(YGX0504)资助

摘　　要：为了提高冬小麦花培苗染色体加倍效率,分别用不同浓度的秋水仙碱对参试的冬小麦材料的花药愈伤组织、再生植株根系和花培苗分蘖节进行了加倍处理。结果表明,用0.02‰和0.05‰秋水仙碱浓度处理的愈伤组织再生植株结实率达33.3%～61.5%。用0.2%的秋水仙碱浸根处理5h,结实株率平均高达37.5%。用0.04%的秋水仙碱1%的二甲亚砜溶液浸泡分蘖节的时间应在5～10h之间较为适宜,结实株率平均可达50%以上。In order to increase chromosome doubling frequency for anther culture of winter wheat, the callus, roots of regenerative plants and tillering node were treated respectively with different concentration of colchicine. The result showed that there were 33.3%~61.5% setting percentage in the treated callus with 0.02‰ and 0.05‰ colchicine soluion. After soaking roots of regenerative plants for 5 hours using 0.02‰ and 0.05‰ colchicine soluion, the average setting percentage reached 37.5%. The setting percentage were over 50% in tillering node soaked for 5~10 hours using 0.04‰ colchicine soluion and 1‰dimethylsulfoxide.

关 键 词：冬小麦 花药培养 愈伤组织 秋水仙碱 染色体加倍 

分 类 号：S512.1[农业科学—作物学]