大豆异黄酮对雄性香猪生殖调控的影响  

EFFECTS OF SOY ISOFLAVONES ON THE MALE REPRODUCTIVE REGULATION IN BLACK SMALL-EARED PIGS

作　　者：袁晓雪[1,2] 李丽立[1] 范觉鑫[3] 肖朝武[4] 印遇龙[1] 

机构地区：[1]中国科学院亚热带农业生态研究所中国科学院亚热带农业生态过程重点实验室湖南省畜禽健康养殖工程技术研究中心,长沙410125 [2]中国科学院研究生院,北京100049 [3]湖南农业大学动物科技学院,长沙410128 [4]加拿大卫生部食品署营养局分子实验室

出　　处：《营养学报》2011年第6期584-588,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30972118);湖南省基金资助项目(No.08JJ3080)

摘　　要：目的探讨大豆异黄酮对雄性香猪生殖调控的影响。方法 50头雄性仔香猪,随机分为正常对照组(饲喂试验日粮),低、中、高剂量大豆异黄酮组和阳性对照组。大豆异黄酮(纯度为80%)125,250,500 mg/kg和己烯雌酚0.5 mg/kg均匀混于饲料中给予,60 d称重后前腔静脉采血,放免法分析促性腺激素释放激素(GnRH)、促黄体激素(LH)、卵泡刺激素(FSH)、睾酮和雌二醇的浓度;采血后处死,取下丘脑、脑垂体、睾丸组织,睾丸和附睾称重,采用荧光定量PCR检测睾丸组织中与睾酮合成相关的酶P450scc、3β-HSD和相关蛋白StAR mRNA表达量的变化。结果 250 mg/kg大豆异黄酮组的睾丸指数比正常对照组提高44.76%,差异显著(P<0.05);血清中睾酮水平比正常对照组提高51.94%,差异显著(P<0.05);睾酮合成调节蛋白StAR mRNA的表达量高达1.43%,与对照组差异显著(P<0.05)。500 mg/kg大豆异黄酮组的睾丸指数比正常对照组降低39.92%,差异显著(P<0.05);血清中睾酮水平比正常对照组降低53.69%,差异显著(P<0.05);StAR mRNA的表达量为0.49%,与250 mg/kg大豆异黄酮组差异显著(P<0.05)。结论大豆异黄酮能影响雄性生殖激素分泌、睾丸和附睾组织的生长发育、睾酮合成相关酶的活性以及大脑中生殖激素基因的表达,并与剂量有关。Objective To evaluate the effects of soy isoflavones （SI） on male reproductive regulation in Black Small-eared pigs. Method Fifty male black small-eared pigs were randomly divided into control group （fed a test diet）, low, medium and high doses of SI group and diethylstilbestrol group. Three different doses of SI （125 mg/ kg, 250 mg/ kg, and 500 mg / kg） and 0.5 mg/kg diethylstilbestrol were evenly mixed in the feed and fed to pigs for 60 d （The purity of SI 80%）. The levels of GnRH, LH, FSH, Tes and E2 were analyzed by radioimmunoassay, and testis and epididymis were weighed. The mRNA expression of P450scc, 3β -HSD and StAR in testicular tissue, which were associated with testosterone synthesis, was measured by RT-PCR. Results In 250 mg/kg SI group, testicular index increased by 44.76% （P〈0.05）; and serum testosterone level increased by 51.49% than the control group （P〈0.05）. The mRNA expression of StAR was up to 1.43%, significantly different to control group （P〈0.05）. In 500 mg/kg SI group, the testicular index decreased by 39.92% （P〈0.05）. and serum testosterone level decreased by 53.69% than the control group （P〈0.05）. The mRNA expression of StAR was 0.49%, significantly different to 250 mg/kg SI group （P〈0.05）. Conclusion Soy isoflavones can affect the male reproductive hormone secretion, the growth and development of testis and epididymis, enzyme activity of testosterone synthesis, and expression of reproductive hormones＇ gene in the brain, and in dosage-dependant ways.

关 键 词：大豆异黄酮 睾丸 生殖激素 睾酮合成 香猪 

分 类 号：S828.5[农业科学—畜牧学]