鸡新城疫重组鸡痘病毒活疫苗的免疫持续期和加强免疫研究  

Duration of immunity and boost vaccination of a recombinant fowlpox virus expressing Newcastle disease virus HN gene

作　　者：甘军纪[1] 陶鸽[1] 张胜文[1] 吴永启[1] 彭大新[1] 刘秀梵[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009

出　　处：《中国兽医学报》2011年第12期1681-1685,共5页Chinese Journal of Veterinary Science

基　　金：国家863计划(2006AA10A205);江苏省高技术研究计划(BG2007336)

摘　　要：本研究旨在评价表达新城疫病毒(NDV)血凝素-神经氨酸酶(HN)基因的重组鸡痘病毒(rFPV-12LSHN)活疫苗的免疫持续期和加强免疫对疫苗免疫效力的影响。用rFPV-12LSHN活疫苗免疫14日龄SPF鸡,103PFU/羽,7d即可检测到NDV HI抗体应答,对NDV强毒F48E8株攻毒保护率达100%。一次免疫18周后,对NDV强毒攻击依然提供完全保护。鸡痘病毒(FPV)疫苗免疫4周,再接种rFPV-12LSHN活疫苗,攻毒保护率降低至50%。相反,rFPV-12LSHN免疫4周,随后二次免疫可显著提高对NDV的体液免疫应答水平(P<0.01),对NDV强毒攻击的保护率仍然为100%。结果表明,表达NDV HN基因的重组鸡痘病毒(rFPV-12LSHN)活疫苗,能够快速建立坚强免疫力,免疫持续期至少可达18周,rFPV-12LSHN的二次免疫可以提高疫苗的免疫力。To evaluate the duration of immunity and the efficacy of boost vaccination of a recombinant fowlpox virus（rFPV-12LSHN） expressing HN gene from a Newcastle disease virus（NDV） isolate（ZJ1）.Two-week old SPF chickens vaccinated with one dose of vaccine containing 103 plaque forming units（PFU） of rFPV-12LSHN were completely protected from virulent NDV after 7 days vaccination,hemagglutination inhibition（HI） antibody against NDV could be detected as early as 7 days postvaccination and immune protection lasted up to 18 weeks postvaccination.The efficacy of different vaccination schedules was evaluated in SPF chickens,the lowest efficacy（50%）were observed in the group of chickens,which was vaccinated with fowlpox vaccine and followed by rFPV-12LSHN vaccine at 4 weeks interval.However,the chickens primed and boosted with rFPV-12LSHN at 4 weeks interval produced a significant increase of the HI antibody against NDV（P0.01）and provided 100% protection against virulent NDV challenge.The result indicated that the fowlpox vector-based vaccine for ND could induce an earlier onset of immunity and a long duration of at least 18 weeks.Furthermore,a second vaccination with rPFV is valuable for induction of optimal immunity.

关 键 词：新城疫病毒 重组鸡痘病毒 疫苗 持续期 加强免疫 

分 类 号：S852.65[农业科学—基础兽医学]