PRRSV ORF6及IL-18基因重组真核质粒试验免疫研究  

Immunogenicity of recombinant plasmid expressing the PRRSV ORF6 and IL-18 genes

作　　者：牛伟 王中华[3] 王晓莉[1] 宋德武[1] 母连志[1] 丁壮[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林农业科技学院,吉林吉林市132000 [3]商丘职业技术学院,河南商丘476100

出　　处：《中国兽医学报》2011年第12期1691-1694,共4页Chinese Journal of Veterinary Science

基　　金：广东省科技攻关重大项目(2008A020100020)

摘　　要：将本实验室构建的重组质粒接种PRRSV的自然宿主断奶仔猪,分析其诱发细胞免疫和体液免疫应答的能力。结果表明:重组质粒pEGFP-ORF6和pEGFP-IL18-ORF6免疫组在首免后14d开始产生特异性抗体,且其产生抗体水平随时间呈上升趋势,但pEGFP-ORF6和pEGFP-IL18-ORF6两组之间产生抗体差异不显著(P>0.05)。中和抗体检测结果显示只有pEGFP-ORF6在首免后42d有低水平的中和抗体产生。细胞免疫检测结果表明,pEGFP-IL18-ORF6诱导T淋巴细胞增殖和促进IFN-γ和IL-2的分泌的能力显著高于pEGFP-ORF6和pEGFP-IL18(P<0.05),表明IL-18有效地发挥了分子其佐剂的效用,能增强细胞免疫应答并介导较好的Th1类免疫反应。The immunogenicity of recombinant plasmids were further evaluated in the piglets by intramuscular injection,which were PRRSV natural host animal.The results indicated the pEGFP-ORF6 and pEGFP-IL18-ORF6 immune groups produced specific antibodies at 14 d post vaccination,and the production of antibodies increased with time,but the antibody levels were not significant（P0.05）between two groups.Neutralizing antibody analysis showed the pEGFP-N1 induced low titer neutralizing antibodies at 42 d post vaccination.However the pEGFP-IL18-ORF6 could enhance cell-meditated immune response and elicit effective Th1 type immune response by stimulating PRRSV-special T lymphocyte proliferation and promoting the secretion levels of IFN-γ and IL-2.It indicated the IL-18 effectively play the part of molecular adjuvant which could enhance the cell-mediated immune response and Th1 type immune response.

关 键 词：猪繁殖与呼吸综合征病毒 ORF6基因 IL-18 实验免疫 

分 类 号：S852.65[农业科学—基础兽医学]