唐山地区蜱传斑点热群立克次体分子流行病学  

Molecular epidemiological of spotted fever group Rickettsia from ticks in Tangshan area

作　　者：邹亚学[1] 贾青辉[1] 刘朋朋[1] 刘全[2] 高宏伟[2] 陈丽凤[1] 

机构地区：[1]河北科技师范学院,河北秦皇岛066000 [2]军事医学科学院军事兽医研究所吉林人兽共患病预防与控制重点实验室,吉林长春130062

出　　处：《中国兽医学报》2011年第12期1729-1732,共4页Chinese Journal of Veterinary Science

基　　金：河北省科技厅指导项目资助(07276916)

摘　　要：根据已发表的长角血蜱16SrRNA序列及斑点热群立克次体外膜蛋白A(OmpA)基因序列设计2对特异性引物,对唐山地区采集的长角血蜱进行PCR检测,并对阳性样本进行测序和序列分析,抽检样本建立分子系统进化树。结果表明,在315份蜱DNA样本中检测出25份阳性样本,阳性率为7.94%;序列分析结果显示唐山地区长角血蜱携带立克次体同处于一个分支,与日本株立克次体同源性最高(93.30%),其次是福建株立克次体(92.11%),黑龙江立克次体绥芬株(90.45%)、虎林株(90.42%)。结论得出唐山地区蜱传斑点热感染较严重,分子进化分析结果显示唐山地区蜱传斑点热群立克次体可能为一新种。Two sets of specific primers,designed according to 16SrRNA for tick and the OmpA gene specific for spotted fever group Rickettsia（SFGR）,were used to amplify 16SrRNA and OmpA gene from DNA samples extracted from Haemaphysalis longicornis in Tangshan area.The positive samples were sequenced and genetically analyzed by establishment of phylogenetic tree.Of 315 samples,25 samples were positive for SFGR and the infection rate reached to 7.94%.Phylogenetic analysis based on nucleotide sequence revealed that all positive strains were related and clustered together with Rickettsia sp.Japanese strain（93.30%）,Rickettsia sp.Fujian strain（92.11%）,Rickettsia sp.suifen strain（90.45%） and Rickettsia sp.Hulin strain（90.42%）.The infection of spotted fever group Rickettsia in Tangshan area was serious,and the bacterium may be a novel species.

关 键 词：长角血蜱 斑点热群立克次体 分子流行病学 

分 类 号：S852.64[农业科学—基础兽医学]