犬脾特异性转移因子的生物学活性检测  

Biological activity detection of specific transfer factor from canine spleen

作　　者：张英[1] 刘一诺[1] 李莉[1] 马强[1] 房希碧[1] 官员[1] 刘松财[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2011年第12期1769-1772,共4页Chinese Journal of Veterinary Science

基　　金：吉林省科技厅科技支撑计划资助项目(3D510S086604)

摘　　要：采用冻融—透析法提取了免疫犬的特异性转移因子(STF),以健康非免疫犬的非特异性转移因子(NSTF)为对照,对2种转移因子的理化学特性和免疫活性进行了检测与分析。结果表明,2种转移因子的理化学性质相似,STF含有的蛋白量低而核酸量高,而NSTF含有的蛋白量高而核酸量低。E-玫瑰花环试验表明,STF和NSTF均可增强小鼠腹腔巨噬细胞的吞噬功能,增加E-玫瑰花环的形成率,表明TF具有免疫增强作用,其中STF的效果明显高于NSTF(P<0.05)。为评价转移因子的免疫活性,首先利用环磷酰胺建立小鼠免疫抑制模型,通过MTT法比较犬脾STF和NSTF对免疫抑制小鼠、生理盐水接种小鼠和正常小鼠的淋巴细胞转化的作用。结果表明犬STF、NSTF对免疫抑制小鼠的外周血淋巴细胞转化均具有明显的免疫增强作用,与PHA对照组相比差异极显著(P<0.01),其中STF免疫增强作用高于NSTF组,但两者不显著(P>0.05)。生理盐水组和正常小鼠的外周血淋巴细胞转化表现与免疫抑制小鼠基本一致,犬脾STF、NSTF和PHA对生理盐水组和正常小鼠的淋巴细胞均表现出一定的免疫增强活性,而且犬脾STF、NSTF免疫增强作用显著高于PHA组(P<0.01)。Transfer factor was extracted from immunized and nonimmunized canine spleen by freeze-thaw-dialysis.The activity of transfer factor extracted was analyzed by the E-rosette test.The results showed that specific TF and nonspecific TF could increase the E-rosette formation.It was showed that TF had immune-enhancing effect,and the specific TF was higher than the nonspecific TF（P0.05）.The immunological activity was determined by MTT colorimetric method.The results indicated that the specific TF and nonspecific TF could improve the transformation of immunosuppressive mice T lymphocytes（P0.01）.the specific TF and nonspecific TF could induce higher response than PHA,and the specific TF was the highest.Just the same as the results in immunosuppressive mice,in the T lymphocytes transformation test of normal mice,the specific TF and nonspecific TF could also stimulate T lymphocyte transformation（P0.01）.

关 键 词：犬 特异性转移因子 非特异性转移因子 E-玫瑰花环 MTT 免疫活性 

分 类 号：S859.797[农业科学—临床兽医学]