GalR2基因的表达对小鼠抑郁症影响的初步探讨  

Effect of GalR2 gene expression on the depression in mice models

作　　者：张丹[1,2] 杨春[1,2] 何永林[1,2] 徐蕾[1,2] 靳志栋[1,2] 张鹏[1,2] 冯鑫[1,2] 

机构地区：[1]重庆医科大学病原生物学教研室,重庆400016 [2]重庆医科大学神经科学研究中心,重庆400016

出　　处：《中国微生态学杂志》2011年第12期1087-1090,共4页Chinese Journal of Microecology

摘　　要：目的建立C57BL/6J小鼠抑郁症模型,探讨GalR2基因表达对小鼠抑郁症的影响。方法脂质体转染重组真核表达质粒pEGFP-GalR2至人宫颈癌细胞系Hela细胞,Western blotting检测GalR2基因在细胞内的表达。参照CUMS和CORT建模方法构建小鼠抑郁症模型。侧脑室注射脂质体包裹的pEGFP-GalR2质粒,观察GalR2基因表达对小鼠抑郁症的影响。结果通过Western blotting鉴定了GalR2基因获得表达。抑郁症模型小鼠体重增加量、摄食量、液体消耗实验中糖水消耗和糖水偏爱百分比均明显下降,纯水消耗显著提高;强迫游泳实验中挣扎时间和游泳时间缩短,不动时间延长。侧脑室注射pEGFP-GalR2后小鼠行为学改变未得到有统计学意义的结果。结论成功鉴定了GalR2基因在细胞内的表达。成功构建C57BL/6J小鼠抑郁症模型。CORT模型造模效果要优于CUMS模型。GalR2蛋白对抑郁症的影响有待后续实验进一步探讨。Objective To establish the C57BL/6J mice model of depression and evaluate the influence of GalR2 gene expression on depression. Method The eukaryotie expression recombinant plasmid pEGFP-CalR2 was transiently transfeeted into Hela cells, and the expression of GalR2 gene was detected by Western blotting. The mice model of depression was constructed by CUMS and CORT. The plasmid pEGFP-GalR2 was injected intraeerebroventricularly, and the influence of GalR2 gene expression on depression was evaluated. The GalR2 gene expression products in Hela cells were detected by Western blotting. Result Body weight, food intake, sugar consumption and the percentage of preference for sugar of the depressed mice were significantly decreased and pure water consumption was increased compared with control group. In forced swim test, the depressed mice spent less time on swimming and struggling, and more time on immobiliting. Behavioral changes after ICV （intraeerebroventricular injection） had no statistically significant results. Conclusion The expression of GalR2 gene in vitro was identified successfully. The mice model of depression has been established successfully. The model of CORT was better than CUMS. The actual effect of GalR2 gene expression on depression must be verified by future experiments.

关 键 词：GalR2基因 抑郁症 动物模型 

分 类 号：R346[医药卫生—基础医学]