纯钛表面梯度生物活性涂层材料生物相容性研究  

Stuely on to biocompatibility of the gradient bioactive coating prepared on titanium

作　　者：叶迅[1] 朱琳[2] 唐光昕[3] 于书卿[1] 王硕[1] 赵继宗[1] 赵元立[1] 

机构地区：[1]首都医科大学附属北京天坛医院神经外科,北京100050 [2]清华大学生物科学与技术系生物膜与膜生物工程国家重点实验室,北京l00084 [3]清华大学机械系,北京l00084

出　　处：《中华神经外科疾病研究杂志》2011年第6期543-547,共5页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(30500520)

摘　　要：目的评价复合氧化及水热沉淀法制备纯钛表面梯度生物活性涂层材料生物相容性和骨诱导能力。方法通过对L929成纤维细胞和M373成骨细胞体外细胞培养进行细胞相容性实验和动物颅骨植入实验评价梯度羟基磷灰石(HA)涂层生物相容性和骨诱导能力。结果涂层表面L929成纤维细胞附着与增殖实验提示多孔的羟基磷灰石表面涂层对细胞的黏附生长有诱导作用,促进细胞在其表面繁殖。体外细胞毒性实验提示各组材料对M373成骨细胞的生长、增值、代谢无不良影响。在细胞接种后期HA试件组的细胞增殖数较其他组有显著增加。颅骨植入实验提示羟基磷灰石涂层组在诱导成骨反应方面优于其它组。结论复合氧化及水热沉淀法制备纯钛表面梯度生物活性涂层材料具有良好的生物相容性和骨诱导能力,值得作为新型颅骨修补材料。Objective To evaluate the biocompatibility and osteoinduction of the composite-preparing gradient bioactive coating on titanium.Methods Cell culture and methyl thiazolyl tetrazoliym（MTT） tests were used for evaluating the cell biocompatibility and osteoinduction of this gradient bioactive coating material.Implanted rabbits skull bone was used for histological and osteoinductional evaluation of the interface.ResultsMore L929 fibro-sarcoma cells attached and proliferated on the HA coating material surface than titanium surface.The M373 osteoblastic cells on gradient layer were the most among all the specimens throughout the period of the study.The implant on skull was stable without solution or separation.Ossointegration had been achieved on the coating surface after one month implanting.More bone was formed on the coating surface than titanium.Conclusion The composite-preparing gradient bioactive coating on titanium had fine biocompatibility and ossointegration.The gradient bioactive coating prepared on titanium was suitable material for skull repair.

关 键 词：颅骨修补材料 钛金属 羟基磷灰石 梯度涂层 生物相容性 骨诱导 

分 类 号：R318.08[医药卫生—生物医学工程] R459.3[医药卫生—基础医学]