破壁灵芝孢子粉对小鼠T细胞淋巴瘤细胞抑制作用  

Inhibitory Effects of Sporoderm-broken Ganoderma Lucidum Spores on T-cell lymphoma

作　　者：陈娟[1] 余英豪[1] 

机构地区：[1]南京军区福州总医院病理科,福州350025

出　　处：《实验动物与比较医学》2011年第6期426-431,共6页Laboratory Animal and Comparative Medicine

基　　金：南京军区医学科技研究“十一五”计划课题资助（072034）;福建省自然科学基金（2010J01221）

摘　　要：目的 研究灵芝孢子粉在体内、外对小鼠T细胞淋巴瘤细胞生长的抑制作用.方法 运用噻唑蓝（MTT）法研究灵芝孢子粉在体外对EL-4细胞的抑制作用;建立MNU诱导小鼠胸腺T细胞淋巴瘤的裸鼠移植瘤模型,利用裸鼠移植瘤研究灵芝孢子粉对胸腺T细胞淋巴瘤细胞的抑制作用,HE染色观察淋巴瘤组织结构的改变.结果 灵芝孢子粉对EL-4细胞的IC50值随作用时间增加而减小,72 h达到最低,为4.76 g/L.体内抑瘤试验显示,灵芝孢子粉剂量为4 g/kg时,抑制率为45.8％,镜下观察,灵芝孢子粉高剂量治疗组肿瘤坏死区域较大,核碎屑、细胞固缩较多.结论 高浓度及高剂量的灵芝孢子粉具有抑制肿瘤细胞生长的作用.Objective To evaluate the inhibitory effects of sporoderm-broken Ganoderma Lucidum Spores on EL-4 cell in vitro, and in vivo, the nude mice model of the subcutaneous transplanting thymic lymphoma induced by MNU was established to evaluate the inhibitory effect. Mdthods The inhibitory effects of Ganoderma Lucidum Spores on EL-4 cell were detected by MTT method in vitro. The models of the subcutaneous transplanting tumor were established by Thymus T-cell lymphoma cells induced by MNU, the inhibitory rates were observed, and the tumor tissues of the nude mice were taken with HE staining. Results With the increasing of time, the IC50 values were decreased, the minimums of which were 4.76g/L after 72 hours. In vivo, the Ganoderma Lucidum spores had certain experiment therapeu- tic anti-tumor action, and the inhibitory rate was 45.8% with the dose of 4g/kg. The morphologic study of Thymus lymphoma cells of Garoderma Lucidum Spoers group showed more necrosis and nucleoli crumb. Conclusion Garoderma Lucidum Spoers could play inhibitory action on lymphoma cell at high concentration and high dosage.

关 键 词：灵芝孢子粉 T细胞淋巴瘤 EL-4细胞 裸小鼠 

分 类 号：R285.5[医药卫生—中药学]