综合手术方法制作犬慢性肾功能衰竭模型  

Establishment of Chronic Renal Failure Model in Beagle Dogs by Integrative Operation

作　　者：林若勤[1] 傅晓晴[1] 杨朝阳[1] 朱龙[1] 李慧勇[2] 

机构地区：[1]福建中医药大学中医学院,福州350108 [2]福建中医药大学成人教育学院,福州350003

出　　处：《实验动物与比较医学》2011年第6期459-461,共3页Laboratory Animal and Comparative Medicine

基　　金：2007年福建省发展改革委员会项目

摘　　要：目的 研究慢性肾功能衰竭氮质血症期中医脾肾虚型比格犬模型的手术造模方法.方法 采用左肾皮质电灼法与肾动脉狭窄结扎法相结合,1周后切除右肾.术后3d及每周检测血尿素氮、肌酐.结果 造模前尿素氮为7.21±0.72 mmol/L,肌酐为82.40±9.42 μtmol/L,造模术后30 d尿素氮为11.22±5.69 mmol/L,肌酐为125.55±68.50 μmol/L（P值均＜0.05）.临床表现为脾肾虚证候.结论 采用左肾皮质电灼法与肾动脉狭窄结扎法相结合,可以造成理想的慢性肾功能衰竭氮质血症期脾肾虚型比格犬模型.Objective To study the surgical method for establishing chronic renal failure model at azotaemic stage in beagle dogs. Methods The integrative operation was preformed by electric cautery of renal cortex and renal artery ligation in left kidney. Right kidney was removed one week later. Blood urea nitrogen and creatinine were detected 3 days after surgery and every week after surgery. Results The urea nitrogen Was 7.21±0.72 mmol/L and the creatinine was 82.40±9.42 μmol/L before surgery, 30 days after surgery was 11.22±5.69 mmol/L and 125.55±68.50 μmol/L respectively. Both of their P- value were less than 0.05. Conclusions Ideal chronic renal failure model at azotaemic stage in beagle dogs can be obtained by the combination of electric cautery of left renal cortex and left renal artery ligation.

关 键 词：慢性肾功能衰竭 肾虚型 比格犬 动物模型 肾皮电灼法 肾动脉结扎 

分 类 号：R692.5[医药卫生—泌尿科学] R-332[医药卫生—外科学]