重组禽流感H5亚型二价灭活疫苗(H5N1 Re-4+Re-5株)抗体消长规律及免疫程序的研究  

Antibody response and vaccination schedule of bivalent killed avian influenza vaccine of H5 subtype(Re-4+Re-5)

作　　者：范根成[1,2] 左青山 王红 刘东 冯阳 李祥瑞[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]青岛易邦生物工程有限公司,山东青岛266032

出　　处：《中国兽医科学》2011年第12期1253-1259,共7页Chinese Veterinary Science

基　　金：国家科技支撑计划项目(2006BAD06A08)

摘　　要：用同一批重组禽流感H5亚型二价灭活疫苗(H5N1Re-5+Re-4株)免疫SPF鸡、不同环境及饲养管理条件下的商品蛋鸡、肉种鸡及商品肉鸡,免疫后连续检测Re-5及Re-4抗体。检测结果显示,二价疫苗的免疫效果良好;免疫剂量、鸡群的饲养管理条件及鸡群所处环境的污染程度与鸡群免疫后的抗体效价及高抗体效价(≥6lb)维持时间均有关系;商品肉鸡的免疫效果相对较差;母源抗体对二价疫苗的免疫效果有一定的影响;Re-4的抗体效价比Re-5高0.4个滴度以上。上述结果表明,鸡群的免疫程序及免疫剂量应根据饲养管理水平、环境污染程度及出栏时间、鸡的体型而定。The antibody changes against Re-5 and Re-4 of SPF chickens,commercial layer,layer breeders,and broiler were observed post the vaccination with bivalent reassortment of H5 subtype avian influenza vaccine.The results indicated that the vaccination could induce high antibody responses.The antibody titer,high antibody titer and sustain time of high antibody titer were closely related to husbandry conditions and environment pollution.The vaccination result on broiler is not so satisfactory.Maternal antibody level influenced the vaccination result.The titer against Re-4 was 0.4 lb higher than that against Re-5.The results suggested that the vaccination program should be decided on the husbandry conditions,environment pollution,marketing time and weight of chickens.

关 键 词：禽流感 二价疫苗 免疫抗体 

分 类 号：S852.43[农业科学—基础兽医学]