胰岛素样生长因子Ⅰ和胰高血糖素样肽Ⅰ对体外培养的脂肪细胞HSL mRNA表达的影响  

Effects of IGF-Ⅰ and GLP-Ⅰon abundance of HSL mRNA of bovine adipocyte cultured in vitro

作　　者：王娅[1] 邓俊良[1] 王哲[2] 左之才[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医科学》2011年第12期1266-1270,共5页Chinese Veterinary Science

基　　金：国家自然科学基金资助项目(3023062);四川省教育厅重点项目(10ZA048);四川农业大学优秀学士毕业论文培育计划项目

摘　　要：为阐明胰岛素样生长因子Ⅰ(IGF-Ⅰ)、胰高血糖素样肽Ⅰ(GLP-Ⅰ)在乳牛脂肪代谢过程中的调控作用,应用荧光定量PCR法检测IGF-Ⅰ、GLP-Ⅰ对体外培养的脂肪细胞激素敏感脂肪酶(HSL)mRNA丰度的影响。结果显示,随着IGF-Ⅰ、GLP-Ⅰ添加浓度的升高,脂肪细胞HSL mRNA表达逐渐下降。结果表明,IGF-Ⅰ、GLP-Ⅰ直接调控脂肪细胞HSL mRNA的表达。In order to investigate the regulation roles of IGF-Ⅰand GLP-Ⅰin the lipometabolism of dairy cows,the effects of them in bovine were observed by the expression level of HSL mRNA of cultured neonatal calf lipocytes using fluorescent quantitative RT-PCR.The expression level of HSL mRNA decreased following with the increasing concentrations of IGF-Ⅰand GLP-Ⅰ in the media.These results indicated that the IGF-Ⅰand GLP-Ⅰcould regulate the mRNA expressions of cultured adipose cells HSL directly.

关 键 词：IGF-Ⅰ GLP-Ⅰ 脂肪细胞 HSLmRNA 

分 类 号：S816.73[农业科学—饲料科学]