地昔尼尔乳剂在猪体内的药动学研究  

Study on pharmacokinetics of dicyclanil emulsion in swine

作　　者：吴开宝[1] 高丽丽[1] 季辉[1] 江善祥[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095

出　　处：《中国兽医科学》2011年第12期1288-1293,共6页Chinese Veterinary Science

摘　　要：为建立高效液相色谱法(HPLC)测定猪血浆中地昔尼尔质量浓度的方法,探讨地昔尼尔乳剂在猪体内的药动学特征,将50g/kg地昔尼尔乳剂以45mg/kg的单剂量为6头健康猪给药(背正中线浇泼),血浆经乙腈提取,采用HPLC测定了血液中地昔尼尔浓度。本试验条件下,地昔尼尔在0.05～20μg/mL内线性良好,r2=0.999,最低检测限为0.01μg/mL;其药时数据符合一级吸收二室模型,主要药动学参数为:t1/2ka=9.836h±0.198h,t1/2α=13.649h±0.124h,t1/2β=20.745h±1.187h,tmax=17.576h±0.216h,Cmax=9.129μg/mL±0.313μg/mL,AUC=450.010μg/mL.h±2.993μg/mL.h。结果表明,该测定方法专属、准确、灵敏,适用于地昔尼尔的药动学研究;地昔尼尔乳剂经皮给药后,在猪体内的吸收、消除速率较慢,达峰浓度高。To establish a simple assay method for dicyclanil by HPLC and to study the pharmacokinetics characteristics of dicyclanil emulsion in healthy swine,dicyclanil emulsion were applied at a single dosage of 45 mg/kg to six swine. The plasma samples were treated by acetonitrile,and HPLC method was used to detect the dicyclanil concentration in plasma. In the experimental conditions, dicyclanil have a good linear relationship when concentration was from 0.05 to 20 μg/mL（r2 =0. 999）, and the detect limit was 0.01μg/mL. The dates were calculated based on 3P97 pharmacokinetics software. The pharmacokinetics of dicyclanil in swine was fitted to two-compartment with first order absorption open model, the main pharmacokinetics parameters were as follows= t1/2ka = 9. 836 h±0. 198 h,t1/2α = 13. 649 h-±-0. 124 h,t/2β=20. 745 h±1. 187 h,tmax=17. 576 h±0. 216 h,Cmax= 9. 129 μg/mL±0. 313 μg/mL,AUC=450. 010 μg/mL· h±2. 993 μg/mL, h. Results showed that the HPLC method was proprietary,accurate and sensitive,and it is suitable for pharmacokinetic studies. Dicyclanil was absorbed and eliminated slowly in swine,with higher concentration in plasma.

关 键 词：地昔尼尔 药代动力学 HPLC 猪 

分 类 号：S859.795[农业科学—临床兽医学]