印楝种仁粗提物体外抗鸭瘟病毒活性的研究  

Study on antivirus activity of crude extract from neem seed kernel against duck plague virus in vitro

作　　者：徐娇[1] 宋旭[1] 殷中琼[1,2] 程安春[1,2] 贾仁勇[1,2] 刘颖彘[1] 叶奎川[1] 邓云夏[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《中国兽医科学》2011年第12期1302-1307,共6页Chinese Veterinary Science

基　　金："十二五"农村领域国家科技计划项目(2011BAD34B03-4);四川省科技支撑计划项目(2010N20043);教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848)

摘　　要：采用索氏提取法及溶剂萃取法,用950mL/L乙醇提取印楝种仁并萃取,分别得石油醚萃取物、乙酸乙酯萃取物及水层。采用细胞病变(CPE)法及四甲基偶氮唑盐(MTT)比色法研究三种萃取物对鸭瘟病毒(DPV)的抑制作用。结果显示,石油醚萃取物、乙酸乙酯萃取物及水层对细胞的最大无毒浓度分别为0.007 8、0.003 9和0.125 0mg/mL;当其浓度为0.000 5、0.000 3及0.156 0mg/mL时,对DPV的抑制率各为13.23%、29.24%和11.72%。结果表明,三种萃取物对DPV均有不同程度的抑制作用,其中乙酸乙酯萃取物效果较好。The three fractions obtained from chloroform extracts of ethanol extract of neem（Azadirachta indica） seed kernel by petroleum ether,ethyl acetate and distilled water were investigated for antivirus activity against duck plague virus（DPV）in vitro.Duck embryo fibroblasts infected with DPV were treated with the drugs we prepared and the effect of antivirus was judged by cytopathic effect（CPE） and 3-（4,5）-dimethylthiahiazo（-z-y1）-3,5-di-phenytetrazoliumromide（MTT） colorimetric method.The results showed that when the concentrations were 0.000 5,0.000 3 and 0.156 0 mg/mL,the virus inhibition rates of the three extracts were 13.23%,29.24% and 11.72%,respectively,and the maximum non-toxic concentrations of the three extractives were 0.007 8,0.003 9 and 0.125 0 mg/mL respectively.

关 键 词：印楝种仁提取物 鸭瘟病毒 抗病毒活性 

分 类 号：S853.74[农业科学—临床兽医学]