启动子区E-box以及周围CpG岛的甲基化与基因节律性表达的关系  

Circadian E-boxes and surrounding CpG islands are free from methylation throughout the day

作　　者：左晓虹[1] 刘姝[1] 林庆玲[1] 李宁[1] 陈彪[1] 蔡彦宁[1] 

机构地区：[1]首都医科大学宣武医院神经生物学研究室,教育部神经变性病重点实验室,北京100053

出　　处：《首都医科大学学报》2011年第6期781-786,共6页Journal of Capital Medical University

基　　金：国家高技术研究发展计划(863计划)(2006AA02A408);国家重点基础研究发展计划(973项目)(2006CB500701);国家自然科学基金(30771064)资助项目~~

摘　　要：目的探讨mPer2、mCry1和mDBP启动子区E-box以及周围CpG岛的甲基化是否影响E-box和BMAL1/CLOCK基因和蛋白相互作用的方式;以及启动子区的甲基化状态是否影响MAP激酶磷酸化酶1(MAP kinase phosphatise 1,MKP1)的组织特异性表达调控。方法分6个时间点取C57 BL/6J小鼠的肝脏和肾脏,提取DNA,通过重硫酸盐处理基因组DNA,以及测序检测E-box和周围CpG岛的甲基化状态。结果 mPer2、mCry1和mDBP启动子区E-box以及周围CpG岛无明显甲基化状态,肝脏和肾脏中MKP1的E-box以及周围CpG岛亦无明显甲基化状态。结论节律基因的启动子区E-box以及周围CpG岛的甲基化不参与节律基因表达的日节律性调控。Objective To study the DNA methylation of E-boxes which may or may not be involved in the protein/DNA interacting and in the MKP1 tissue-specific expressions. To study the DNA methylation of E-boxes which may or not involved in the protein/DNA interacting and in the MKP1 tissue-specific expressions. Methods C57 BL/6J male mice were randomized into 6 groups and were sacrificed at six different time points during a 24-hour period. Liver and kidney were isolated. Genomic DNA was extracted and DNA methylation was examined using bisulfite sequencing analysis. Results All E-boxes and their surrounding CpG islands were free from methylation. The methylation status of the E-box in the MKP1 promoter in different tissues was also negative. Conclusion Our results indicated that DNA methylation does not determine distinct binding features of circadian genes and also does not regulate the tissue specific clock control of MKP1.

关 键 词：时钟基因 E-BOX CPG岛 DNA甲基化 

分 类 号：Q751[生物学—分子生物学]