α-突触核蛋白在人脑膜瘤组织中的表达  

Immunolocalization of α-synuclein in human meningiomas tissues

作　　者：吴杨[1] 苏玉金[2] 李昕[1] 刘光伟[1] 李尧华[1] 于顺[1] 

机构地区：[1]首都医科大学宣武医院神经生物学研究室,教育部神经变性病重点实验室,北京100053 [2]首都医科大学天坛医院神经科学研究所,北京100050

出　　处：《首都医科大学学报》2011年第6期793-797,共5页Journal of Capital Medical University

基　　金：国家自然科学基金(81071014);国家重点基础发展计划计划(2011CB504101);国家高技术研究发展计划(2006AA02A408);北京市属高等学校人才强教计划资助项目(PHR200907113);北京市自然科学基金(7102076)~~

摘　　要：目的研究α-突触核蛋白(α-synuclein,α-syn)在人脑膜瘤组织和对照脑组织中的表达及其与微管蛋白之间的关系。方法用抗人α-syn抗体对人脑膜瘤组织和对照脑组织进行免疫组织化学染色,观察、比较α-syn在脑膜瘤和对照脑组织中的表达和分布。用免疫荧光组织化学方法检测α-syn和β-微管蛋白以及突触素是否共存。结果对照脑组织中,α-syn主要存在于神经元的末梢和胞质部分,分别与突触素和β-微管蛋白共存。人脑膜瘤组织中,α-syn主要存在于肿瘤细胞的胞质,并且与β-微管蛋白在胞质共存,少部分存在于细胞核中。结论α-syn存在于脑膜瘤细胞的胞质和核中,并与β-微管蛋白在胞质中共存。Objective To investigate the localization of α-synuclein（α-syn） in human brain meningiomas tissues and its association with β-tubulin. Methods Immuohistochemistry with antibodies against α-syn was used to reveal the localizations of α-syn in normal brain and meningiomas tissues.Double immunofluorescent labeling was used to observe the co-localization of α-syn with β-tubulin. Results In normal brain tissues,α-syn was localized in presynaptic terminals and cytoplasms of the neuronal somata,which was co-localized with synaptophysin and β-tubulin,respectively.In meningiomas tissues,α-syn was observed in cytoplasms and nuclei of the tumor cells.α-Syn in tumor cells were found to be co-localized with β-tubulin. Conclusion α-Syn was localized in the cytoplasm and nucleus of the meningiomas cells,and colocalized with β-tubulin.

关 键 词：Α-突触核蛋白 Β-微管蛋白 脑膜瘤 

分 类 号：R338[医药卫生—人体生理学]