玉米SSR连锁图谱构建和粗缩病抗性QTL的初步定位  

SSR Linkage Map Construction and QTL Identification for Resistance Gene of MRDV in Maize

作　　者：商伟[1] 张彦军[1] 魏海忠[1] 孔晓民[2] 蒋飞[2] 刘保申[1] 

机构地区：[1]作物生物学国家重点实验室/山东农业大学农学院,山东泰安271018 [2]济宁市农业科学研究院,山东济宁272031

出　　处：《山东农业科学》2011年第12期1-6,共6页Shandong Agricultural Sciences

基　　金：转基因生物新品种培育重大专项(2009ZX08003-013B);山东省良种产业化项目[鲁科农字(2010)117号];农业生物资源创新利用研究子任务"专用玉米种质创新与利用";山东省科技发展计划项目(2009GG10009021)资助

摘　　要：以玉米自交系80007和80044为亲本衍生的RIL群体构建连锁图谱,对205个F5∶6家系成株期的粗缩病抗性进行鉴定。所构建的连锁图谱共拟合173个SSR标记位点,覆盖基因组919.87 cM,标记间平均距离为5.32 cM。应用完备区间作图法(ICIM)进行QTL分析,结果表明:在泰安环境下检测到5个QTLs,分布在第1、2和第5染色体上,可分别解释4.69%～17.74%的表型变异;在肥城环境下检测到3个QTLs,分布在第1和第2染色体上,可分别解释4.95%～9.13%的表型变异。其中,定位于第2染色体的QTL在两个环境下均被检测到,分别解释抗粗缩病表型变异的17.74%和8.76%,可做进一步精细定位和克隆。A mapping population consisting of 205 F5∶6 recombinant inbred lines（RILs） derived from the cross between maize inbred lines 80007 and 80044 was used in this study.A genetic linkage map was constructed containing 173 SSR markers,which covered 919.87 cM of the maize genome with an average distance of 5.32 cM between markers.The results showed that five QTLs were identified for MRDV resistance using inclusive composite interval mapping method（ICIM）,which were located on chromosome 1,2 and 5 in Taian and explained a range of phenotypic variation from 4.69% to 17.74%;three QTLs for MRDV resistance were mapped on chromosome 1 and 2 in Feicheng,explaining phenotypic variation ranging from 4.95% to 9.13%.One common QTL detected on chromosome 2 was identified in both environments,explaining 17.74% and 8.76% of the phenotypic variation respectively,which could be used for fining mapping and positional cloning.

关 键 词：玉米 SSR 连锁图谱 数量性状位点(QTL) 粗缩病 

分 类 号：S513.032[农业科学—作物学]