新疆牛巴贝斯虫病的流行病学调查  

Epidemiologic Survey for Detection of Babesia bovis-Infected Cattle in Xinjiang by an Improved MSA-2c Indirect ELISA Assay

作　　者：简子健[1] 马素贞[1] 孙其喆[1] 沈炯玉[1] 吕伟[1] 苗中秋[1] 

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052

出　　处：《新疆农业科学》2011年第11期2129-2133,共5页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目(3066141)

摘　　要：【目的】2006～2008年对全疆14个地州的牛巴贝斯虫病进行流行病学调查。【方法】使用已建立的牛巴贝斯虫病间接ELISA检测方法,以纯化的重组蛋白GST-MSA-2作为抗原。【结果】(1)新疆存在着牛巴贝斯虫病。在2006年采集的278份牛血清样品中,阳性血清7份,感染率为2.52%。在2007年的532份牛血清样品中检出阳性血清32份,感染率为3.13%。在2008年的530份牛血清中检出阳性血清43份,感染率为5.28%。(2)2008年在地方流行性疫病区牛巴贝斯虫感染率高达28%。(3)牛巴贝斯虫病所在的地州由2006年的5个扩大到2008年的11个。【结论】新疆牛巴贝斯虫病的感染率逐年上升,疫区面积不断扩大,流行区内感染率激增,牛巴贝斯虫病的防治不容忽视。这是新疆首次利用血清学方法对全疆牛巴贝斯虫病进行大规模的流行病学调查。[ Objiective and Method] The purpose of this project was to investigate the epidemiology of Babesia boris - infected cattle in Xinjiang by the improved indirect MSA - 2c enzyme - linked immunosorbent assay （indirect MSA -2c ELISA）. [ Result] The resuks showed that （1） bovine babesiosis caused by Babesia boris was present in Xinjiang which was the first time to be confirmed by the serological method. The infection rates for Babesia boris from the tested cattle in 2006, 2007 and 2008 were 2.25% （7/278）, 3.31% （32/ 532） and 5.28% （43/530）, respectively; （2） The rate of Babesia boris infection was increased to 28% （ 14/ 50） in some endemic regions where the disease occurred in 2008 ; （3） The number of infected prefectures in Xinjiang were also increased from 5 in 2006 to 11 in 2008. [ Conclusion ] The features of bovine babesiosis caused by Babesia boris were that the infection rate and epidemic regions were increased year by year. It was a question which could be not disregarded. This was a first large - scale epidemiological survey for detection of specific antibodies against Babesia boris in cattle from Xinjiang by an improved indirect NSA -2c ELISA.

关 键 词：牛 牛巴贝斯虫 MSA-2c间接酶联免疫吸附试验 流行病学调查 感染率 

分 类 号：S852.7[农业科学—基础兽医学]