一株具有抗菌作用的P. polymyxa sp.菌株的分离鉴定  

The isolation and identification of a P. polymyxa sp. serain and its bio-control function against pathogenic fungus

作　　者：陈林[1] 董兆麟[1] 段康民[1] 

机构地区：[1]西北大学生命科学学院/西部资源与现代生物技术省部共建教育部重点实验室,陕西西安710069

出　　处：《西北大学学报（自然科学版）》2011年第6期1010-1013,共4页Journal of Northwest University（Natural Science Edition）

基　　金：青海省自然科学基金资助项目(UH0507)

摘　　要：目的研究对辣椒疫病具有防治作用的一株桔抗细菌。方法从辣椒植株根的韧皮部分离出对辣椒疫霉(Phytophora capsic Leonian)具有强烈拮抗作用的辣椒植株内生细菌菌株,通过形态观察、生理生化实验、16s rRNA基因测序以及与NCBI(National Center for Biotechnology Informa-tion)的数据库中进行对比,鉴定该菌株。采用平皿对峙法测定其抑菌活性。结果分离出的该菌株确定为Peanibacillus polymyxa sp(其后简称为J),其对辣椒疫霉(phytophora capsic Leonian)、西瓜枯萎(Fusarium oxysporum f.sp.niveum)、小麦赤霉(Gibberella saubinetii(Mont.)Sacc.)、马铃薯干腐(Gibberella pulicris)、烟草赤星(A.alternata(Fr.)Keisslerf.sp.nicotianae)和番茄早疫(Alternariasolani)病害真菌具有抑菌活性。结论对Paenibacillus polymyxa sp.的生物学特性研究结果表明,菌株对多种植物病原真菌具有抑制作用。同时,它还可固定大气中的氮,且无磷酸酶、纤维素酶和脂肪酶活性,它同宿主属互惠共生关系。因此,该菌株可能是适用于生物防治的一株优良菌株。Aim Characterization of a Paenibacillus polymyxa strain J that was isolated from the stem of the capsicum tree, beneath the epidermis. Methods 16S rRNA gene sequence of this isolate was analyzed and blasted against the NCBI （National Center for Biotechnology Information） database, and the morphological, biochemical and physiological characteristics of this strain were investigated. Resnlts The strain was identified as P. polymyxa sp. It showed strong growth inhibition against pathogenic fungi and bacteria. The plants pathogenic fungi inhibited include phytophora capsic Leonian, Fusarium oxysporum f. sp. niveum, Gibberella saubinetii （Mont.） Sacc. , Gibberella pulicris ,A. alternata （ Fr. ） Keisslerf. sp. nicotianae and Alteruaria solani. Conclusion This strain not only exhibits broad spectrum of antagonism but also has the ability of fixing N2. It does not produce phosphatase, cellu- lase or lipase activity. There is a mutualistic relationship between Paenibacillus polymyxa J and the host. Therefore, this strain is a potential biocontrol agent.

关 键 词：PeanibacilIus polymyxa 对峙法 拮抗作用 内生细菌 

分 类 号：Q939.92[生物学—微生物学]