基因枪法遗传转化双孢蘑菇菌褶  

Transformation of Agaricus bisporus by Particle Bombardment of Fruit Body Gill Tissue

作　　者：曹育博[1] 刘天翔[1] 周巍巍[1] 高玉千[1] 戚元成[1] 申进文[1] 邱立友[1] 

机构地区：[1]河南农业大学生命科学学院,农业部农业微生物酶工程重点实验室,河南郑州450002

出　　处：《食用菌学报》2011年第3期9-11,共3页Acta Edulis Fungi

基　　金：河南省科技攻关项目(编号:082102150048)的部分研究内容

摘　　要：以双孢蘑菇(Agaricus bisporus)2793为试材,采用基因枪法对其菌褶进行遗传转化,相对转化率为2.69%,转化子经PCR检测表明,外源潮霉素B抗性基因已经被转入到菌丝体中。Transformation of Agaricus bisporus by particle bombardment of fruit body gill tissue was confirmed using the hygromycin B gene as a selective marker. PCR amplification revealed that DNA containing the resistance marker had been inserted into fungal hyphae with a transformation efficiency of 2.69%.

关 键 词：双孢蘑菇 基因枪 遗传转化 潮霉素B抗性 

分 类 号：S646.11[农业科学—蔬菜学]