山莨菪碱对烫伤大鼠血浆内毒素及肿瘤坏死因子-α水平的影响  

The effect of anisodamine on plasma endotoxin and tumor necrosis factor-α levels in scalded rats

作　　者：孙晓庆[1] 付小兵[1] 晋桦[1] 孙世荣[1] 姚咏明[1] 

机构地区：[1]解放军第三○四医院创伤研究室,北京100037

出　　处：《中国危重病急救医学》2000年第2期73-73,共1页Chinese Critical Care Medicine

基　　金：国家杰出青年科学基金!(39525024)

摘　　要：目的：探讨山莨菪碱改善创伤和烧伤后全身炎症反应综合征的作用机制。方法：雄性Ｗｉｓｔａｒ大鼠背部２５％ ～３０％ 深Ⅱ～Ⅲ度烫伤，烫伤后分别给予５％ 葡萄糖盐水或山莨菪碱，检测血浆内毒素（ＬＰＳ）和肿瘤坏死因子 α（ＴＮＦ α）的浓度。结果：大鼠血中ＬＰＳ烫伤后８～１６ 小时升高，２４ 小时即恢复正常；而血中ＴＮＦ α的升高表现为双峰状，第１ 个高峰出现在伤后２ 小时，第２ 个高峰出现在伤后１６～２４ 小时，均与血中ＬＰＳ的升高无明显关系。山莨菪碱无论对伤后２ 小时还是伤后２４ 小时的ＴＮＦ α高峰均有明显的降低作用，但不影响血中的ＬＰＳ水平。结论：烫伤后ＴＮＦ α升高并不完全依赖于血浆ＬＰＳ浓度的变化，山莨菪碱降低烫伤后血浆ＴＮＦ α水平可能是其改善创伤和烧伤后全身炎症反应综合征和脓毒症的作用机制之一。Objective:To investigate the potential mechanisms underlying beneficial effect of anisodamine treatment on systemic inflammatory response syndrome following burns.Methods:Male Wistar rats were subjected to a 25%30% of total body surface area scalded injury.After burns,animals were intraperitoneally injected with 5% glucose saline solution or anisodamine.Plasma levels of endotoxin (LPS) and tumor necrosis factorα (TNFα)were measured.Results:It showed that plasma LPS increased at 8 to 16 hours postburn,tending to recover at 24 hours later.Elevation of plasma TNFα levels in scalded rats manifested two peaks,the first appeared at 2 hours postburn,and the second at 16 to 24 hours postburn.No marked correlation was found between plasma LPS and TNFα levels at any time point.Anisodamine effectively reduced the elevation of TNFα at both 2 hours and 16 to 24 hours after thermal injury.Conclusions:Our results suggest that the elevation of plasma TNFα levels were independent on the plasma levels of LPS,anisodamine might be an effective agent in reducing plasma TNFα levels and attenuating systemic inflammatory response syndrome following burns.

关 键 词：烫伤 内毒素 山莨菪碱 TNF-Α 

分 类 号：R965[医药卫生—药理学]