地沟油中胆固醇的LC/MS/MS定性定量检测  

Test of Qualitative and Quantitative Method for Cholesterol in Hogwash Oil by LC/MS/MS

作　　者：余雯静[1] 郑利[1] 沈崇玉[2] 

机构地区：[1]苏州出入境检验检疫局,苏州215104 [2]江苏出入境检验检疫局,南京210001

出　　处：《重庆理工大学学报（自然科学）》2011年第12期41-45,共5页Journal of Chongqing University of Technology：Natural Science

摘　　要：利用LC/MS/MS测定地沟油中胆固醇含量。研究过程中采用MRM-IDA-EPI的增强子离子扫描模式对样品进行分析,得到数据谱库检索,根据匹配度确证化合物的真伪,排除假阳性的可能,并且实现一次进样同时得到胆固醇的定量、定性结果。实验结果表明,该方法的最低定量限为50 ng/mL,通过添加不同基质得到回收率为82%～93%。从结果中可看出与地沟油相比新植物油中胆固醇含量很低,所以若利用胆固醇作为地沟油的一个衡量指标,将为地沟油的确证提供有效依据。Cholesterol content was detected with LC/MS/MS method for identification of hogwash oil, and set up EPI （ enhance product ion） MS library. LC/MS/MS limit of quantification （LOQ） based on a signal/noise ratio of 10, was estimated to be for 50ng/mL. The MRM-IDA-EPI has shown the quantification and confirmation analysis in one injection, and the overall average recovery in cholesterol spiked oil as 82% ~93%. Compared with the hogwash oil,new oil was in a low quantity, so this method is well used for detection the cholesterol in the hogwash oil.

关 键 词：地沟油 胆固醇 增强子离子扫描 液相色谱质谱联用仪 

分 类 号：TQ645[化学工程—精细化工]