补肾密骨液对骨代谢影响的体外实验研究  

Extracorporeal Experimental Study of the Effect of Bushen Migu Ye on Bony Metabolism

作　　者：胡光亮[1] 杜靖远[2] 王洪[2] 谢晶[2] 

机构地区：[1]青岛市市立医院,山东青岛266011 [2]同济医科大学附属协和医院

出　　处：《中国骨伤》2000年第2期83-85,共3页China Journal of Orthopaedics and Traumatology

摘　　要：目的　探讨补肾密骨液影响骨代谢的作用机制。方法　利用组织培养技术,将不同浓度的补肾密骨液与分离培养制得的新生大鼠颅盖骨成骨细胞样细胞共同培养,用MTT比色分析法和3HTdR掺入法观察补肾密骨液对成骨细胞样细胞增殖的影响,用原子吸收分光光度法观察补肾密骨液对颅盖骨吸收的影响,并与鲑鱼降钙素进行对照。结果　补肾密骨液以剂量依赖的方式促进成骨细胞样细胞的增殖,并抑制颅盖骨的吸收;而鲑鱼降钙素对成骨细胞样细胞的增殖无影响,但对颅盖骨的吸收有明显的抑制作用。结论　补肾密骨液直接参与骨代谢的调节,促进骨形成,同时抑制骨吸收。Objective To investigate the mechanism of action of Bushen Migu Ye(BSMGY)on bony metabolism Methods Using tissue culture technology,the suspension of osteoblast like cells which obtained from new born mouse skullcap and BSMGY of different concentration were cultured together The effect of BSMGY on proliferation of osteoblast like cells was analysed by the colorimetric analytical method and 3H TdR incorporation method The effect of BSMGY on bone absorption was studied with atomic absorption spectropholometry Salmon calcitonin acted as comparison Results BSMGY directly promoted proliferation of osteoblast like cells at a dose dependent manner and inhibited bone absorption;while salmon calicitonin had no effect on proliferation of osteoblastic cells,but inhibited bone absorption obviously Conclusion BSMGY directly affect bony metabolism by promoting bone formation and inhibiting bone absorption

关 键 词：中草药 药理学 成骨细胞 补肾密骨液 骨代谢 

分 类 号：R285.5[医药卫生—中药学]