吉非替尼脂质体的制备及含量测定  

Preparation of gefitinib liposomes and determination of its content

作　　者：何琳[1] 李哲[1] 马艳铃[1] 邓意辉[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2012年第1期1-4,30,共5页Journal of Shenyang Pharmaceutical University

摘　　要：目的制备吉非替尼(gefitinib,GFB)脂质体,建立GFB含量测定方法。方法采用乙二胺四乙酸铵(ammonium ethylenediaminetetraacetate,NH4EDTA)梯度法制备吉非替尼脂质体(gefitinib lipo-some,GFBL),以紫外分光光度法测定GFB的含量,检测波长为350 nm。结果实验条件下所用辅料对GFB的测定无干扰,GFB质量浓度在6.0～14.0 mg.L-1内线性关系良好(r=0.999 8),回收率在100.3%～101.9%内,日内及日间RSD均小于2%(n=3),制得GFBL的平均包封率为78.1%。结论乙二胺四乙酸铵梯度法可用于制备GFBL;紫外分光光度法简便易行,结果准确,可用于GFBL的含量测定。Objective To prepare gefitinib liposomes and establish a method for determination of gefitinib in the liposomes. Methods Gefitinib liposomes were prepared by ammonium ethylenediaminetetraacetate gradient method. Ultraviolet spectrophotometry was used for analyzing the amount of gefitinib, and the detection wavelength was set at 350 nm. Results Gefitinib had a good linear relation over the range of 6. 0- 14. 0 mg·L^-1（ r =0. 999 8） ,the intra-day RSD and inter-day RSD were less than 2% ,the recovery of gefitinib was in a range of 100. 3%- 101.9%. The average entrapment efficiency of gefitinib liposomes were 78.1%. Conclusions Ammonium ethylenediaminetetraacetate gradient method, can be used to prepare ge- fitinib liposomes. It is a simple and accurate method to determine the contents of gefitinib in the liposomes by ultraviolet spectrophotometry.

关 键 词：吉非替尼 脂质体 乙二胺四乙酸铵梯度法 紫外分光光度法 含量测定 

分 类 号：R94[医药卫生—药剂学] R917[医药卫生—药学]