RP-HPLC法测定刺五加中紫丁香苷和异秦皮啶的含量  

Determination of syringin and isofraxidin in Acanthopanax senticosus(Rupr.et Maxim.) Harms by RP-HPLC

作　　者：袁霞[1] 刘唯芬[1] 张艳君[1] 

机构地区：[1]沈阳药科大学高等职业技术学院,辽宁沈阳110026

出　　处：《沈阳药科大学学报》2012年第1期36-38,44,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立高效液相色谱法测定刺五加中紫丁香苷和异秦皮啶的含量。方法采用KromasilC18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈(A)-体积分数为1%乙酸水溶液(B),梯度洗脱;流速为1.0 mL.min-1;检测波长为265 nm(0 min),341nm(11 min)。结果紫丁香苷和异秦皮啶质量浓度分别在26.0～435 mg.L-1(r=0.999 7)和0.270～4.50 mg.L-1(r=0.999 6)内与峰面积呈良好的线性关系;平均回收率分别为98.01%(RSD=1.2%,n=6)和97.53%(RSD=1.1%,n=6)。结论该方法可同时测定刺五加药材中紫丁香苷和异秦皮啶的含量。Objective To develop an HPLC method for determination of syringin and isofraxidin in Acanthopanax senticosus by RP-HPLC. Methods The chromatographic separation was carried out on a Kromasil C18 colunm（250 mm × 4. 6 mm,5 μm）, The mobile phase consisted of acetonitrile and 1% acetic acid with gradient elution was used; the flow rate was 1.0 mL· min ^- 1. The UV detector was set at 265 nm （ 0 rain） and 341 nm（ 11 min）. Results A good linear relationship of syringin was in the range of 26. 0-435 mg·L^-1 （ r = 0. 999 7 ）and that of isofraxidin was in the range of 0. 270-4. 50 mg·L^-1 （ r = 0. 999 6）. The average recoveries of syringin and isofraxidin were 98.01% （RSD = 1.2% ,n =6）and 97.53% （RSD = 1.1% ,n =6） ,respectively. Conclusions The method is suitable for the simultaneous determination of syringin and isofraxidin in Acanthopanax senticosus.

关 键 词：高效液相色谱法 刺五加 紫丁香苷 异秦皮啶 含量测定 

分 类 号：R917[医药卫生—药物分析学]