2-甲基-β-环糊精作为手性选择剂对5种药物对映体的毛细管电泳拆分研究  

Chiral separation of five basic drug enantiomer by capillary electrophoresis with 2-O-methylated-β-cyclodextrin

作　　者：马晓伟[1] 左莉华[1] 徐淑英[1] 郭兴杰[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2012年第1期55-58,共4页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(30973675)

摘　　要：目的以2-甲基-β-环糊精(2-O-methylated-β-cyclodextrin,2-O-M-β-CD)为手性添加剂,利用毛细管电泳法(capillary electrophoresis,CE)对消旋体药物甲溴后马托品、氧氟沙星、酮康唑、利阿唑和卡维地洛进行拆分研究。方法考察了背景电解质溶液的pH值、2-O-M-β-CD浓度、缓冲盐浓度对分离的影响,优化了分离条件。结果在最佳分离条件下,甲溴后马托品、氧氟沙星、酮康唑、利阿唑对映体达到完全分离,分离度分别为3.6、2.8、1.7、1.5;卡维地洛对映体分离度达到1.4。结论所建立的毛细管电泳法适于除卡维地洛外其余4种药物的对映体分离。Objective To establish a capillary electrophoresis （CE）method for separating homatropine methyl bromide, ofloxacin, ketoconazole, liarozole and carvedilol. Methods 2-O-methylated-β-cyclodextdn （ 2-O-M-β-CD） was used as a chiral selector. The influence of buffer pH, 2-O-M-β-CD concentration, buffer concentration on separation was investigated. Results Under the conditions tested, homatropine methylbromide, ofloxacin,ketoconazole, liarozole, carvedilol were separated with the maximum resolutioln factors of 3.6, 2.8,1.7,1.5,1.4, respectively. Conclusions This CE method for separating enantiomers has advantages of the short analysis time, good reproducibility and high sensitivity.

关 键 词：2-甲基-β-环糊精 毛细管电泳 对映体分离 

分 类 号：R917[医药卫生—药物分析学]