UV-B诱导对离体阔叶十大功劳叶次生代谢产物累积影响的研究  

Study on Influence of UV-B Induction on Accumulation of Secondary Metabolites in Postharvest Leaves of Mahonia bealei(Fort.) Carr.

作　　者：宁伟[1,2] 章燕珍[2] 卢晓萍[2] 王伟[4] 翟兴英[4] 王晶[3] 田景奎[2] 彭欣[1] 张琳[2] 

机构地区：[1]山东中医药大学理工学院,济南250355 [2]浙江大学生物医学工程与仪器科学学院,教育部生物医学工程重点实验室,杭州310027 [3]山东中医药大学生理生化学系,济南250355 [4]江西中医学院药学院,南昌330004

出　　处：《中国药学杂志》2012年第1期19-22,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(30873468)

摘　　要：目的考察UV-B诱导对于离体阔叶十大功劳叶中3种生物碱为主的次生代谢产物含量的影响。方法以高效液相色谱法建立UV-B诱导前后叶片中次生代谢产物的色谱图,通过比较分析,得到化合物含量的变化情况。结果与对照组比较,UV-B诱导组次生代谢产物的含量变化显著,药根碱与巴马汀含量显著增加,均在诱导6 h后达到峰值,其中药根碱增幅410.84%,巴马汀增幅达118.76%,小檗碱诱导后含量则显著降低。结论 UV-B诱导对离体阔叶十大功劳叶中次生代谢产物含量累积有显著影响。OBJECTIVE To investigate the influence of UV-B induction on the content of secondary metabolites, three kinds of alkaloid especially, in post-harvest leaves of Mahonia bealei （ Fort. ） Carr.. METHODS The HPLC chromatograms of secondary metabolites in leaves before and after UV-B induction were established, and the content changes of secondary metabolites were obtained by means of comparison and analysis. RESULTS In comparison with the control group, the contents of the secondary metabolites after UV-B induction changed dramatically. The contents of jatrorrhizine and palmatine increased obviously and reached a plateau after UV- B induction for six hours. The amplification of jatrorrhizine was 410. 84% and that of palmatine reached 118.76% , but the content of berberine decreased obviously after induction. CONCLUSION UV-B induction has remarkable effects on accumulation of secondary metabolites in postharvest leaves of Mahonia bealei （ Fort. ） Cart.

关 键 词：UV—B诱导 阔叶十大功劳 次生代谢产物 

分 类 号：R282[医药卫生—中药学]