LC-MS^n鉴定肝毒性吡咯里西啶类生物碱千里光碱大鼠体内代谢产物  

Identification of Metabolites of Senecionine,a Hepatotoxic Pyrrolizidine Alkaloid,by Liquid Chromatography/Tandem Mass Spectrometry

作　　者：熊爱珍[1] 杨莉[1] 张芳[1] 何芋岐[1] 王长虹[1] 王峥涛[1,2,3] 

机构地区：[1]上海中医药大学中药研究所中药标准化教育部重点实验,上海201203 [2]上海中医药大学中药研究所上海市复方中药重点实验室,上海201203 [3]中国药科大学生药学研究室,南京210038

出　　处：《中国药学杂志》2012年第1期54-60,共7页Chinese Pharmaceutical Journal

基　　金：“重大新药创制”科技重大专项(2009ZX09308-003,2009ZX09052-021,2009ZX09052-024);国家自然科学基金(30701104);上海市国际合作基金项目(09540704700)

摘　　要：目的研究肝毒性吡咯里西啶类生物碱千里光碱的大鼠体内代谢。方法 SD大鼠口服千里光碱,收集胆汁、尿液及粪便样本,采用液相色谱-多级质谱联用对代谢产物进行研究,推导其体内代谢模式。结果正负离子下共检测到38种代谢产物,主要为千里光碱及其代谢物的氮氧化物,羟化产物,水解产物,以及硫酸结合物,葡萄糖醛酸结合物和谷胱甘肽结合物。另外,尿液、胆汁与粪便中检测到的代谢物有一定差异,胆汁和尿液中代谢产物较多。结论千里光碱在大鼠体内代谢广泛,形成多种代谢产物。OBJECTIVE To investigate the metabolic profile of senecionine in rats. METHODS Liquid chromatography-tandem mass spectrometry（LC-MSn） mass spectrometry was used for the analysis of senecionine and its metabolites in rats bile, urine, and feces after a single oral dose of senecionine. RESULTS In total 38 metabolites were identified, including oxidation products, by-droxylation products, hydrolysis products, sulfation products, glucuronidation products, and GSH-conjugations. And metabolites were found to be more abundant in bile and urine than those in feces. CONCLUSION Senecionine undergoes extensive metabolism in rats.

关 键 词：吡咯里西啶类生物碱 千里光碱 多级质谱 体内代谢 

分 类 号：R965[医药卫生—药理学]