采用分子表面印迹技术构建手性空穴实现对天冬氨酸对映体的识别拆分  

STUDIES ON REALIZATING CHIRAL SEPARATION OF ENANTIOMERS OF ASPARTIC ACID BY CONSTITUTING CHIRAL CAVES WITH SURFACE-MOLECULAR IMPRINTING TECHNIQUE

作　　者：郭建峰[1] 高保娇[1] 

机构地区：[1]中北大学化工与环境学院,太原030051

出　　处：《高分子学报》2012年第1期47-55,共9页Acta Polymerica Sinica

基　　金：山西省自然科学基金(基金号2008021013);山西省研究生优秀创新项目(项目号20103085)资助

摘　　要：在溶液聚合体系中,采用"接出"法("graft from"method)将功能单体4-乙烯基吡啶(4VP)接枝聚合于微米级硅胶表面,制得了功能接枝微粒P4VP/SiO2,采用本课题组建立的新型分子表面印迹技术,以天冬氨酸(Asp)的一种对映体L-Asp为模板分子,二溴己烷为交联剂,对接枝在硅胶表面的大分子P4VP实施了分子印迹,制备了L-Asp分子表面印迹材料MIP-P4VP/SiO2.以天冬氨酸另一种对映体D-Asp为对比物,采用静态与动态两种方法深入研究了MIP-P4VP/SiO2对L-Asp分子的识别性能,重点探索了采用MIP-P4VP/SiO2对天冬氨酸两种对映体进行拆分的可行性.实验结果表明,印迹材料MIP-P4VP/SiO2对模板分子L-Asp具有良好的识别选择性与结合亲和性,相对于D-Asp,识别选择性系数为3.85,对天冬氨酸的外消旋体具有良好的拆分性能.印迹材料MIP-P4VP/SiO2也具有良好的解吸性能,以稀NaOH水溶液作为洗脱液,12个床体积内解吸率即可达到98.52%.4-Vinyl pyridine （4VP） was grafted on micron-sized silica gel particles in a manner of ＂graft from＂ in a solution poltmerization system by right of the mediation of the coupling agent 3- methacryloxypropyltrimethoxysilane （ MPS ） , obtaining the grafted functional particles P4VP/SiO2. Subsequently, the molecular imprinting towards the grafted P4VP was conducted with L-Asp that is one enantiomer of aspartic acid （Asp） as template molecule and 1,6-dibromohexane as crosslinking agent by using the novel surface-molecular imprinting technique put forward and established by our research group,and the L- Asp molecule-imprinted material MIP-P4VP/SiO2 was prepared. With another enantiomer of aspartic acid, D- Asp,as a contrast compound, both static method and dynamic method were adopted to study the recognition character of MIP-P4VP/SiO2 for L-Asp, and the separation ability of MIP-P4VP/SiO2 towards L-Asp and D- Asp in the raceme was explored. The experiment results show that MIP-P4VP/SiO2 have obvious recognition selectivity and fine binding affinity for L-Asp, and the selectivity coefficient is equal to 3.85 with respect to D- Asp, displaying the fine separation property for the enantiomers of aspartic acid. Besides, MIP-P4VP/SiO2 has excellent elution property,with a diluted aqueous solution of NaOH as the eluent, the desorption ratios of L-Asp reach 98.52% in 12 BV.

关 键 词：天冬氨酸 手性拆分 分子表面印迹 4-乙烯基吡啶 接枝聚合 分子识别 

分 类 号：O621.2[理学—有机化学]