细胞因子体外扩增脐血CD_(34)^+细胞移植SCID鼠人类免疫功能的建立  

Engraftment of severe combined immunodeficient mice transplanted with ex vivo expanded human CD_(34 )~ + cord blood cells

作　　者：仇志根[1] 范华骅[1] 高峰[1] 

机构地区：[1]上海市血液中心输血研究所,上海市200051

出　　处：《临床血液学杂志》2000年第1期13-14,共2页Journal of Clinical Hematology

摘　　要：目的：探讨扩增的脐血ＣＤ３４细胞体内造血功能。方法：ＦＬＴ３ 配体联合血小板生成素体外培养脐血ＣＤ＋３４细胞２～３ 周后移植ＳＣＩＤ鼠，采用ＦＣＭ 检测ＳＣＩＤ鼠骨髓、外周血、脾脏中的人类ＣＤ４５、ＣＤ３、ＣＤ１９抗原。结果：脐血ＣＤ＋３４细胞扩增１０～３７ 倍，移植６．６×１０４～６．９×１０５ 脐血ＣＤ＋３４细胞于ＳＣＩＤ鼠，６ 周后１０ 只ＳＣＩＤ鼠骨髓中均检测到人类ＣＤ４５抗原（０．２％ ～４．０％ ），６ 只ＳＣＩＤ鼠外周血、脾脏中检测到人类ＣＤ３ 抗原（０．１％ ～２．３％ ），３ 只ＳＣＩＤ鼠中外周血、脾脏中检测到人类ＣＤ１９抗原（０．３％ ～１．３％ ）。结论：ＦＬＴ３ 配体联合血小板生成素体外扩增的脐血ＣＤ＋３４细胞能重建ＳＣＩＤ鼠人类免疫功能。Objective:To explore the hematopoietic function of cytokine expanding CD + 34 cells from umbilical cord blood in vitro.Method:Cord blood cells expanded with FLT3(FMS like tyrosine kinase 3) ligand and thrombopoietin were transplanted in SCID(severe combined immune deficient)mice. Human mature cell surface markers were detected with flow cytometry and used as indicator of succeeding transplantation.Result:After 2 and 3 weeks culture,the number of CD + 34 cells increased approximately 10 and 37 folds. After transplantation with 6.6 ×10 4 to 6.9 ×10 5 CD + 34 cells,respectively the percentage of human CD + 45 cells in the bone marrow of all 10 SCID mice is 0.2 %～ 4.0 %. The percentage of human CD + 3 cells in the peripheral blood and spleen of 6 SCID mice is 0.1 %～ 2.3 %. The percentage of human CD + 19 cells in the peripheral blood and spleen of 3 SCID mice are 0.3 %～ 1.3 %.Conclusion:CD + 34 cells expanded with FLT3 ligand and thrombopoietin are potential engraft used for reconstitution of both hematopoietic and immunologic function of SCID mice.

关 键 词：脐血 体外扩增 移植 SCID鼠 

分 类 号：R457[医药卫生—治疗学]