基于纳米银与有机多孔材料/纳米金修饰的甲胎蛋白免疫传感器研究  

Amperometric immunosensor for α-1-fetoprotein based on nano-Ag and organic porous materials/gold nanoparticles composite

作　　者：牟青松[1,2] 袁若[1] 柴雅琴[1] 洪成林[1] 

机构地区：[1]西南大学发光与实时分析教育部重点实验室,化学化工学院,重庆400715 [2]遵义师范学院化学系,遵义563002

出　　处：《分析试验室》2012年第1期16-20,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20675064);重庆自然科学基金项目(CSTC-2005BB4100)资助

摘　　要：在金电极表面电沉积银为氧化还原探针,利用有机多孔材料(PTC-NH2)、纳米金(nano-Au)固载甲胎蛋白抗体(anti-AFP),制备出用于检测甲胎蛋白(AFP)的安培型免疫传感器。通过交流阻抗技术、循环伏安法研究了电极的电化学特性,考察了孵育时间、测试液pH值等实验条件对传感器性能的影响,并利用扫描电子显微镜(SEM)对电极的修饰过程进行了表征。该传感器对AFP有良好的电流响应,线性范围分别为1.0～20.0ng/mL和20.0～60.0 ng/mL,检测限为0.6 ng/mL。A new highly sensitive amperometric immunosensor was developed for the detection of a-1-fetoprotien（AFP） based on deposition AgNO3 assembly of 3,4,9,10-perylenetetracarboxylic acid-bound thionine molecules（PTCTH） and gold nanoparticles（GNPs） modified gold electrode surface by electrostatic adsorption.The electrochemical properties of the electrode were studied by using electrochemical impedance spectroscopy（EIS） and cyclic voltammetry（CV）.The factors influencing the performance of the immunosensor,including the incubation time and the solution pH value,were studied in detail.The modified process was also characterized by scanning electron microscope（SEM）.The immunosensor was highly sensitive to AFP and the linear range was from 1.0 to 20.0 ng/mL and from 20.0 to 60.0 ng/mL,respectively.

关 键 词：免疫传感器 甲胎蛋白 纳米金 纳米银 

分 类 号：O657.1[理学—分析化学]