野生与栽培羌活药材挥发油含量及组分的比较分析  

Comparison analysis of contents and constituents of volatile oils extracted from wild and cultivated rhizoma et radix notopterygii

作　　者：李春丽[1,2] 周玉碧[1] 周国英[1,3] 孙菁[1,3] 杨路存[1,2] 徐文华[1,3] 

机构地区：[1]中国科学院西北高原生物研究所,西宁810001 [2]中国科学院研究生院,北京100049 [3]中国科学院高原生物适应与进化重点实验室,西宁810001

出　　处：《分析试验室》2012年第1期29-34,共6页Chinese Journal of Analysis Laboratory

基　　金：中国科学院"西部之光"人才培养计划项目(2007年);科技部科技人员服务企业行动项目(2009GJG20016)资助

摘　　要：分析野生和栽培羌活药材挥发油含量及其组分的变化,为羌活药材的引种栽培及进一步的开发利用提供了依据。采用水蒸气蒸馏法提取羌活药材中的挥发油,通过GC-MS对挥发油成分进行分析鉴定;利用聚类分析对测定结果进行分类;通过相关分析研究海拔对羌活药材挥发油含量的影响。6份药材样品挥发油含量范围为1.60～7.98 mL/100g,6份羌活药材挥发油成分经GC-MS分析,共鉴定出57个化合物,共有成分12个;基于挥发油成分种类及相对含量的聚类分析显示了羌活药材挥发性成分种类及含量的种间差异;相关分析结果表明,海拔高度与挥发油含量存在显著正相关。野生和栽培羌活药材挥发油含量存在差异,总体上野生羌活药材的挥发油含量高于栽培种;羌活药材挥发油含量随海拔升高而增加。To determine the contents and constituents of volatile oils extracted from wild and cultivated Rhizoma et Radix Notopterygii,which can provide experimental foundation for reasonable utilization of Rhizoma et Radix Notopterygii.Volatile oils were extracted by steam distillation.The chemical constituents were separated and identified by gas chromatography-mass spectrometry（GC-MS）.The relative content of each component was determined by area normalization.The classification was carried out by using the Hierarchical clustering analysis.The correlation analysis was used to study the effect of altitude on the contents of volatile oil.The contents of volatile oil in wild and cultivated Rhizoma et Radix Notopterygii ranged from 1.60% to 7.98%.The content was highest in the wild perennial samples collected from county town of Ban Ma.Fifty seven constituents were identified by GC-MS analysis,and there were twelve same compounds in the six samples.According to the result of classification,all samples collected were divided into two grades.The result of correlation analysis showed that the altitude had positive significant influence on the contents of volatile oil in Rhizoma et Radix Notopterygii.The contents of volatile oil in the wild samples were basically higher than those in the cultivated samples.After cultivated,the constituents and their relative contents in the volatile oil had some changes.But the main compounds of the volatile oil were the same between the wild and cultivated samples.The proportion of the mutual components was high.With the increase of altitude,the contents of volatile oil showed the tendency of increase.

关 键 词：野生 栽培 羌活 挥发油 组分分析 

分 类 号：O657.63[理学—分析化学]