邻硝基苯基荧光酮与头孢唑啉的荷移反应研究  

Study on charge-transfer reaction between o-nitro phenyl fluorone and cefazolin

作　　者：安娜[1] 敖登高娃[1] 于彦珠[1] 

机构地区：[1]内蒙古大学化学化工学院,呼和浩特010021

出　　处：《分析试验室》2012年第1期102-104,共3页Chinese Journal of Analysis Laboratory

基　　金：内蒙古自然科学基金项目(2009MS0206);内蒙古大学创新人才培养项目(2-1;2.1-042)资助

摘　　要：采用分光光度法,以邻硝基苯基荧光酮(O-NPF)为显色剂,研究了O-NPF与头孢唑啉(CFZ)络合物的可见光谱性质及测定条件。结果表明:CFZ在4.545～72.72μg/mL范围内遵循比尔定律,回归方程为:A=0.668ρ+0.5341,检出限1.53μg/mL,表观摩尔吸光系数为5.41×104L.mol-1.cm-1,相关系数为R2=0.9891,并探讨了其反应机理。方法已用于尿样及生物样品中CFZ含量的测定。The visible spectral characterist ic and the reaction condition of the complex of o-nitro phenyl fluorone（O-NPF） and cefazolin（CFZ） were investigated by spectrophotometry with O-NPF as a developer.The results showed that the concentration of CFZ obeyed the Beer＇s law in the range of 4.545～72.72μg /mL and the regression equation was A=0.668ρ＋0.5341 with the correlation coefficient r of 0.9891,the apparent molar absorptivity was 5.41×104 L·moL-1·cm-1and the detection limit was 1.53μg /mL.Further more,the reaction mechanism had been explored.The proposed method has been successfully applied to the determination of CFZ in urine and biological samples,and the results were satisfying.

关 键 词：邻硝基苯基荧光酮 头孢唑啉 分光光度法 

分 类 号：O657.3[理学—分析化学]