免疫细胞联合腺病毒Ad205-IL-15对肝癌细胞的杀伤研究  

Anti-Tumor Efficacy by Immune Cells Combined with Adenovirus Ad205-IL-15 in HepatOcelIuIar Carcinoma Cells

作　　者：徐科[1] 胡贲[1] 苏虹[1] 胡徐庞[1] 刘佳[1] 杨智[1] 刘立[1] 钱程[1] 魏旭斌[1] 

机构地区：[1]浙江理工大学新元医药研究所,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2012年第1期99-102,共4页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：国家自然科学基金国际合作重大项目(81020108026)

摘　　要：在已有腺病毒Ad205-IL-15的基础上尝试与过继免疫细胞CIK、NK92等联合开展对肝癌细胞的杀伤性研究。首次通过荧光化学发光的原理构建出携带表达萤光素酶报告基因的肝癌细胞系Huh7-Luc、MHCC97H-Luc来检测细胞的存活率。结果表明:免疫细胞联合Ad205-IL-15对Huh7-Luc、MHCC97H-Luc两株肝癌细胞表现出了较高协同杀伤作用。证明将免疫细胞与腺病毒联合可以相互弥补各自治疗的不足之处,提高了抗肿瘤效果。同时,由于操作方便,荧光素酶报告基因测定法也为检测细胞存活率提供了新的技术手段。To study the anti-tumor effects of immune cells （CIK, NK92） in combination with adenovirus Ad205-ID15 on liver cancer, the authors construct and examine the expression of luciferase reporter gene in HuhT.MHCC97H cell lines, and then using fluorescent chemical light successfully the authors detect the anti-cancer effects of immune cells, oncolytic adenovirus Ad205-IL15, and combination of two agents respectively. The results show that combination of immune cells and AdCN205-IL-15 increases antitumor efficacy in vitro compared to either alone. At the same time, the luciferase reporter gene assay, due to ease of operation, also provides a new detection technological means of cell viability.

关 键 词：腺病毒Ad205-1L-15 CIK NK92 过继免疫 荧光素酶报告基因 

分 类 号：Q786[生物学—分子生物学]