糖皮质激素对哮喘小鼠CD4^+CD25^+调节性T细胞和TLR4表达的影响  

Effect of glucocorticoid on CD4^+CD25^+ regulatory T cells and the expression of TLR4 in asthmatic mice

作　　者：庞英[1] 李敏[1] 张剑波[2] 姚斌[1] 

机构地区：[1]四川省医学科学院四川省人民医院儿科,四川成都610072 [2]四川省医学科学院四川省人民医院医学中心实验室,四川成都610072

出　　处：《实用医院临床杂志》2012年第1期60-62,共3页Practical Journal of Clinical Medicine

基　　金：四川省卫生厅科研基金(编号:20070089)

摘　　要：目的探讨糖皮质激素对哮喘小鼠CD4+CD25+调节性T细胞(regulatory T cells,Tr)及Toll样受体4(TLR4)表达的影响。方法将雌性BALB/c小鼠30只按随机数字表法分成正常对照组(NS)、哮喘组(OVA)和哮喘糖皮质激素治疗组(OVA/GC)制备动物模型,每组10只。取小鼠左肺组织作病理切片观察炎症改变;同时应用流式细胞检测技术检测各组小鼠脾单个核细胞CD4+CD25+Tr占CD4+T细胞的百分率及CD4+CD25+Tr上的TLR4平均荧光强度。结果哮喘小鼠脾单个核细胞CD4+CD25+Tr百分率明显降低(P<0.01),但该细胞上表达的TLR4平均荧光强度值与正常对照组差异无统计学意义(P>0.05);经糖皮质激素治疗后哮喘小鼠CD4+CD25+Tr百分率和该细胞上表达的TLR4平均荧光强度均明显升高,差异有统计学意义(P<0.01)。结论糖皮质激素可促进哮喘小鼠CD4+CD25+Tr数量的增加及TLR4的表达,可能是治疗哮喘的免疫学机制之一。Objective To discuss the effect of glucocortieoid on CD4^＋ CD25^＋ regulatory T cells （Tr） and the expression of TLR4 in asthmatic mice. Methods Thirty female BALB/c mice were randomly assigned to the control group （ NS ）, the asthma group （OVA） and the glucocorticoid group （OVA/GC） ,with 10 mice in each group. Tissues of left lung were obtained from mice to observe pathological changes. Flow cytometry was used to detect the percentage of CD4^＋CD25^＋ Tr in CD4^＋T cells and the mean fluorescence intensity of TLR4 on CD4^＋CD25^＋ Tr, and isolate the splenic mononuclear cells. Results The percentage of CD4^＋ CD25^＋Tr in group OVA decreased significantly （P 〈 0.01 ）. Difference in mean fluorescence intensity of TLR4 on CD4^＋CD25^＋ Tr was not significant between group OVA and group NS （P 〉 0.05 ）. After glucocorticoid treatment, the percentage of CD4^＋ CD25^＋Tr and the mean fluorescence intensity of TLR4 on CD4^＋ CD25 ^＋ Tr significantly increased in asthmatic mice （ P 〈 0.01 ）. Conclusion Glucocorticoids can increase the number CD4^＋ CD25 ^＋ Tr, promote the expression of TLR4 in asthmatic mice, which may be one of the immunological therapies for asthma.

关 键 词：哮喘 CD4+CD25+调节性T细胞 TOLL样受体4 糖皮质激素 小鼠 

分 类 号：R977.11[医药卫生—药品] R562.25[医药卫生—药学]