氮胁迫对水稻营养生长期氮代谢及相关基因表达量的影响  

Effects of nitrogen deficiency on nitrogen metabolism and expression of genes related during vegetative growth stage of rice

作　　者：罗凤[1] 卢永恩[1] 杨猛[1] 练兴明[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2012年第1期16-22,共7页Journal of Huazhong Agricultural University

基　　金：国家863项目(2010AA101802);国家重点基础研究发展计划(2011CB100304)

摘　　要：通过对水稻品种日本晴进行0、1h和1、3、7d的缺氮胁迫,以及缺氮7d后,恢复供氮生长2h和1d,研究氮素同化相关酶的基因表达及其活性的动态变化情况。结果表明:缺氮胁迫下,根部NH4+、NO3-含量显著下降。短期缺氮胁迫下,地上部NR1、NR2、NiR2、GS2、Fd-GOGAT、GDH2、GDH3以及根部NR1、NR2、GDH4的表达量均有增加;随着缺氮胁迫时间延长,上述基因的表达量均大幅下降。缺氮胁迫下,植株GS、Fd-GOGAT、地上部NR和根部NADH-GOGAT的活性下降,地上部NADPH-GDH活性增加,根部NR、GDH活性先增加后下降,地上部NiR活性先下降后增加。植株缺氮7d后,恢复供氮生长1d时,NR、NiR、GS、GOGAT、GDH的基因表达量及活性基本趋于恢复正常水平,部分基因表达量有所增加。Nitrogen is one of essential nutrients for plant growth. Under the N deficiency at different time points, the dynamic changes of the expression of genes and activity of enzymes related to nitrogen assimilation in rice were investigated. The results showed that the concentrations of ammonium and ni- trate significantly reduced under the N deficiency. Under the N deficiency for a short time, the expression level of genes including NR1, NR2, NiR2, GS2, Fd-GOGAT, GDH2, GDH3 in the shoots as well as NR1,NR2,GDH4 in the roots were up-regulated,and then their expression level decreased after a long time under the N deficiency. Along with the extension of time under the N deficiency, the GS, Fd- GOGAT activities in whole plants, NR activity in shoots and NADH-GOGAT activity in roots decreased while the NADPH-GDH activity in shoots increased. In roots the activities of NR, GDH increased at first and then decreased,while in shoots the NiR activity was just the opposite. Re-supplied N after 7 days of N deficiency,the transcriptional expression level and enzyme activities of NR, NiR, GS, GOGAT, GDH restored, and expression level of some genes increased as well.

关 键 词：水稻 氮胁迫 基因表达 氮代谢 营养生长期 

分 类 号：S143.1[农业科学—肥料学] S511.506.2[农业科学—农业基础科学]