超氧化物歧化酶与苏云金芽胞杆菌高温诱导产黑色素相关  

Superoxide dismutase and the melanin production in Bacillus thuringiensis induced by high temperature

作　　者：刘风侠[1] 杨文君[1] 阮丽芳[1] 孙明[1] 

机构地区：[1]华中农业大学生命科学技术学院/农业微生物学国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2012年第1期39-43,共5页Journal of Huazhong Agricultural University

基　　金：国家高技术研究发展计划("863"计划)(2011AA10A203863);国家重点基础研究发展计划(973计划)项目(2009CB118902)

摘　　要：为探讨苏云金芽胞杆菌高温诱导产黑色素机制,利用含mini-Tn10转座子载体pIC333构建苏云金芽胞杆菌油松亚种4CA1,随机插入突变体库,以寻找相关调控基因。通过高温诱导筛选得到1株在42℃产黑色素减弱的菌株;克隆得到mini-Tn10中断基因的部分序列,通过测序及同源性比对分析,确定中断基因为超氧化物歧化酶基因sodA2。Bacillus thuringiensis subsp, yosoo 4CA1 can not produce melanin at 28 ℃,while it may possess high yield of melanin when induced at 42 ℃ In order to study the mechanism of melanin produc- tion induced by high temperature, a random insertional mutant library of B. thuringiensis subsp, yosoo 4CA1 was constructed with plasmid piC333 containing the mini-Tn10 transposon. Compared with the wild-type strain 4CA1,a mutant of producing melanintion weakly at 42 ℃was obtained. Then the flan- king sequence of mini-Tn10 was cloned and sequenced. The results showed that the gene interrupted by mini-Tn10 was sodA2.

关 键 词：黑色素 转座子 突变体库 超氧化物歧化酶(SOD) 苏云金芽胞杆菌 

分 类 号：Q781[生物学—分子生物学]