紫外线照射对棉铃虫成虫几种同工酶的影响  

Effects of ultraviolet light irradiation on several isozymes in Helicoverpa armigera(Hüber) adults

作　　者：孟建玉[1,2] 张长禹[2] 雷朝亮[2] 

机构地区：[1]贵州省烟草科学研究所,贵阳550081 [2]华中农业大学植物科学技术学院/湖北省昆虫资源利用与害虫可持续治理重点实验室,武汉430070

出　　处：《华中农业大学学报》2012年第1期69-72,共4页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30871639)

摘　　要：采用同工酶电泳的方法,研究了紫外(ultraviolet,UV)胁迫条件下对棉铃虫体内酯酶、过氧化物酶(peroxidase,POX)及过氧化氢酶(catalase,CAT)等同工酶的影响。结果表明:与对照相比,紫外线照射处理组酯酶同工酶谱带发生了质和量的变化,照射时间为30min和60min时,谱带E4、E9、E10增强,谱带E2、E8减弱,谱带E1、E5、E7、E11消失,新增了谱带E3、E6;照射时间延长至90min时,谱带E4、E9增强,谱带E2、E8减弱,谱带E1、E5、E7消失,新增了谱带E3、E6。紫外线照射处理下,棉铃虫POX同工酶谱带P5有所增强,CAT同工酶谱带的变化因照射时间不同而有所差异。与对照相比,紫外线照射处理组的C1谱带增强,但C2谱带在30min时减弱,60min时恢复到对照水平,90min时与对照相比又有所减弱。The effects of ultraviolet （UV） light stress on esterase, peroxidases （POX）,and catalase （CAT） isozymes in Helicoverpa armigera （Hiiber） adults were studied by isozyme eleetrophoresis. When exposed to UV light irradiation, zymogram of esterase isozyme changed mainly in number and activity of isozyme. After 30 min and 60 min exposure, the intensity of isozyme bands E4, E9 and El0 were enhanced,E2 and E8 were weakened. The bands E1,E5,E7 and Ell disappeared after UV light irradiation, while E3 and E6 newly emerged. At the longest exposure time （90 min）, the intensity of isozyme bands E4 and E9 was enhanced, while the intensity of E2 and E8 was weakened. The bands E1, E5 and E7 disappeared after UV light irradiation, whereas E3 and E6 newly emerged. The intensity of POX band P5 was enhanced in adults following the exposure to UV light for 30,60,90 minutes. The intensity of CAT band C1 was enhanced in adults following the exposure to UV light for 30, 60,90 mi- nutes, but that of band C2 was weakened after 30 min and 90 min exposure in comparison with the con- trol.

关 键 词：棉铃虫 紫外光 照射 同工酶 

分 类 号：S435.622.2[农业科学—农业昆虫与害虫防治]