柚皮苷的高效液相色谱定量分析  

Quantitative Determination of Naringin by High Performance Liquid Chromatography

作　　者：宁方刚[1] 许建中[2] 李小波[3] 陈清西[1] 

机构地区：[1]厦门大学生命科学学院,厦门361005 [2]国家海洋局第三海洋研究所,厦门361005 [3]厦门大学化学化工学院化学实验教学中心,厦门361005

出　　处：《天然产物研究与开发》2011年第B12期86-89,共4页Natural Product Research and Development

基　　金：福建省科技计划重点项目（2010Y0035）

摘　　要：建立快速灵敏检测柚皮苷的高效液相色谱法（HPLC）。该方法采用Betasil C18色谱柱，以甲醇-水-冰醋酸为流动相来检测柚皮苷含量。分析结果表明：柚皮苷溶液在1．53—153．30mg／L范围内浓度与峰面积线性关系良好，相关系数r≥0．9999；方法的检测限为0．30ng；精密度实验表明连续6次进样柚皮苷峰面积的相对标准偏差（RSD）为0．94％；加标回收率在100．90％-107．14％。稳定性实验表明在4℃下放置8d，柚皮苷的降解率均小于3．78％；在30cC下放置8d，柚皮苷的降解率均小于4．85％。本方法简单、可靠、灵敏，可用于柚皮苷的精密定量分析。To develop and validate a sensitive and specific high performance liquid chromatography （HPLC） method for the determination of naringin. Naringin was analyzed by a Betasil C18 column using 40：60 （v/v） methanoL/water as eluent which contained 0.06% acetic acid by this method. The method for analyzing naringin was proved to be accurate and precise with the linearity range being 1.53 to 153, 30 mg/L. The linear correlation coefficient r≥0.9999 and the limit of detection （LOD） for the method was 0.30 ng. The precision experiment,carried out by injecting the same naringin sample for six times, showed that the relative standard deviation （RSD） for the six peak areas was 0.94%. The recovery rate was 100.90%-107.14%. The stability experiment indicated that the degradation rates of naringin were all less than 3. 78% and 4.85% within 8 days when stored at 4℃ and 30℃ ,respectively. The method established was simple,accurate and sensitive. It is suitable to analyze naringin using HPLC method.

关 键 词：高效液相色谱法 柚皮苷 检测限 精密度 加标回收率 稳定性 

分 类 号：O657.7[理学—分析化学]