积雪草总苷提取工艺研究  

Extraction Process of Total Saponins from Centella asiatica

作　　者：贾广韬[1] 吕秀阳[1] 

机构地区：[1]浙江大学化学工程与生物工程学系,杭州310027

出　　处：《天然产物研究与开发》2011年第B12期106-111,共6页Natural Product Research and Development

摘　　要：本研究建立了一种以羟基积雪草苷为对照品、利用比色法测定积雪草总苷含量的方法，通过实验确定比色法的反应条件为香草醛浓度5％、高氯酸用量0．8mL、反应温度60℃、反应时间15min，稳定性考察发现反应结束后2h内吸光度基本不变。研究了浸渍法提取积雪草总苷的工艺，确定提取条件为70％乙醇水溶液为溶剂，料液比为1：10，室温下提取3次，每次提取时间为4h，此条件下积雪草总苷的收率为8．96％。另外，积雪草不同部位总苷含量的差异研究表明，叶片中总苷的含量最高（6．12％），茎部次之（2．43％），根部含量最少（1．11％）。In this study, a colorimetric method to detect the content of total saponins from Centella asiatica was established, and the reaction conditions were as follows：the concentration of vanillin was 5%, the content of perchloric acid was 0.8 mL,reaction temperature and time was 60 ℃and 15 min,respectively. After the reaction,the absorbance could maintain a stable status in 2 h. Secondly, a dipping method to extract total saponins from CenteUa asiatics was optimized by the single factor and the orthogonal test. The optimal extraction conditions were achieved as follows：the solvent was 70% ethanol-water solution;the ratio of material and solvent volumes was 1： 10;extraction times were 3 times at room temperature and each extraction process lasted for 4 h, and under this condition, the recovery of total saponins was 8.96%. Finally, the contents of total saponins of different part of Centella asiat/ca were tested. According to the results, the content of total saponins in the leaf of Centella asiatica was the highest （6.12%） ,then in the stick （2.43%） ,and the lowest in the root （ 1.11% ）.

关 键 词：积雪草总苷 比色法 提取工艺 

分 类 号：R284.2[医药卫生—中药学]