Na~＋(K~＋)/H~＋转运蛋白NHX基因的研究进展  

Progress of Research on Na^+(K^+)/H^+ Antiporter NHX Gene

作　　者：李静[1] 刘明[1] 孙晶[1] 李思楠[1] 李文滨[1] 武小霞[1] 

机构地区：[1]东北农业大学农学院,大豆生物学教育部重点实验室,黑龙江哈尔滨150030

出　　处：《大豆科学》2011年第6期1035-1039,共5页Soybean Science

基　　金：国家自然科学基金资助项目(3107438);黑龙江省教育厅课题资助项目(11551048);转基因生物新品种培育重大专项资助项目(2011ZX08004-002);东北农业大学博士后基金与博士启动基金资助项目(2009RC47)

摘　　要：Na+(K+)/H+转运蛋白是液泡膜中的一种离子反向运输体,是生物界普遍存在的负责Na+(K+)/H+交换的一种跨膜运输蛋白。Na+(K+)/H+逆向转运蛋白由NHX家族基因调控表达。过表达NHX家族基因能提高植物中Na+(K+)/H+逆向转运蛋白的活性。从而调节细胞质内pH值、维持Na+(K+)浓度、K+/Na+比、保持细胞膨压、控制细胞扩增的功能,是植物耐盐的关键因子。该文主要对Na+(K+)/H+逆向转运蛋白的发现、功能以及调控其表达的NHX基因的克隆、分类及其与抗旱耐盐之间的关系进行了综述,旨在全面了解其功能和作用机理后将其转入到大豆基因组中,获得抗旱耐盐的转基因大豆种质。Na＋（K＋）/H＋antiporter are ubiquitous membrane proteins which catalyze the exchange of Na＋（K＋）for H＋ across membranes and play important roles in controlling cellular pH,Na＋homeostasis cell turgor pressure and cell proliferation.In particular,the vacuolar Na＋（K＋）/H＋ antiporter has been investigated and proposed to play an important role in salt tolerance.NHX gene control the expression of Na＋（K＋）/H＋ antiporter,overexpress NHX gene in plants can improve the activity of Na＋（K＋）/H＋ antiporter.In the study of plant stress tolerance,more and more researchers have paid attention to Na＋（K＋）/H＋ antiporter.NHX gene which control expression of Na＋（K＋）/H＋ antiporter were found in many crops,and proved its relationship with the resilience.In this study,the found and functions of Na＋（K＋）/H＋ antiporter,together with cloning and classification of NHX gene and the relationship between drought tolerance were reviewed.In order to obtain the transgenic soybean with drought resistance,understanding of function and action mechanism made a foundation for soybean NHX gene transformation.

关 键 词：Na+(K+)/H+转运蛋白 NHX基因 研究进展 

分 类 号：S565.1[农业科学—作物学]