弥漫性脑创伤后Bcl-2家族与线粒体途径细胞凋亡的相关性分析  

Correlation analysis between Bcl-2 family and mitochondria approach apoptosis following traumatic brain injury

作　　者：王金林[1] 尹立国[2] 李军 

机构地区：[1]唐山市工人医院,河北唐山063000 [2]遵化市人民医院 [3]玉田县第二医院

出　　处：《中国实验诊断学》2011年第12期2001-2003,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨脑创伤后Bcl-2家族在线粒体途径细胞凋亡中的作用。方法采用改进的Marmarou’s法建立大鼠中度闭合性弥漫性颅脑损伤模型。应用免疫组化,TUNEL法对大鼠伤后皮质细胞色素c、Bax、Bcl-2表达及凋亡细胞数进行动态观察。结果伤后1 h至24 h Cytc表达与TUNEL阳性细胞数密切相关(r=0.958,P<0.05);伤后1 h至24 h Bax和Bcl-2二者的比例与Cytc免疫反应相关(r=0.996,P<0.01),但Cytc表达峰值(24 h)早于Bax和Bcl-2二者的比例高峰值(48 h);伤后1 h至72 h Bax和Bcl-2二者的比例与TUNEL阳性细胞数密切相关(r=0.925,P<0.01)。结论 Bax和Bcl-2二者的比例对Ctyc释放的调节起重要作用,Bax和Bcl-2二者的比例在Ctyc释放后的凋亡基因的调节中可能仍发挥重要的作用。Objective To investigate the effect of Bcl-2 family in mitochondria approach apoptosis following traumatic brain injury Methods The impeoved model of moderate closed brain injury was established in rats by using of the method found by Marmarou.After traumatic brain injury,cyt-c bax bcl-2 and countity of apoptosis neural cell in the cortex of rats was observed dynamically with the method of immunohistochemry and TUNEL.Results The immunoreaction of Cytc was related to the quantity of TUNEL positive cells from 1h to 24 h（r=0.958,P0.05）;The ratio of Bax and Bcl-2 was related to the immunoreaction of Cytc from 1h to 24h（r=0.996,P0.01）,but the peak of the immunoreaction of Cytc（24h） was earlier than that of the ratio of Bax and Bcl-2（48h）;The ratio of Bax and Bcl-2 was related to the quantity of TUNEL positive cells from 1h to 72h（r=0.925,P0.01） after traumatic brain injury.Conclusion The ratio of Bax and Bcl-2 was important in regulation of Cytc release and in modulation of apoptosis gene expression after Cytc release

关 键 词：颅脑创伤 凋亡 CYTC 线粒体膜通透性转换孔 BCL-2家族 

分 类 号：R651.15[医药卫生—外科学]