木质素降解菌株产木质素降解酶的研究  

Studies on Ligninase production by Lignin-degrading fungus

作　　者：燕红[1,2,3] 张晓甜[1] 刘明[1] 

机构地区：[1]哈尔滨理工大学化学与环境工程学院,哈尔滨150040 [2]黑龙江省农业科学院博士后科研工作站,哈尔滨150086 [3]东北林业大学生物学博士后科研流动站,哈尔滨150040

出　　处：《黑龙江大学自然科学学报》2011年第6期845-847,852,共4页Journal of Natural Science of Heilongjiang University

基　　金：2008年度黑龙江省教育厅科学技术面上项目(11531045)

摘　　要：采用液体静止培养,通过对分离筛选获得的两株高效木质素降解菌株15-1和14-7的发酵产木质素酶条件进行优化,得出结论:两株菌的最佳碳源均为稻草,最佳氮源均为尿素,最佳温度均为30℃。在最佳培养条件下进行发酵产酶,测得14-7的木素过氧化物酶和锰过氧化物酶活力分别为0.350 U.mL-1和0.080 U.mL-1;而15-1的木素过氧化物酶和锰过氧化物酶活力分别为0.300 U.mL-1和0.072 U.mL-1。Optimal culture condition for production of ligninase using two high ligninolytic activity strains （termed 14-7 and 15 -1, respectively）are, investigated. Ligninase activities of static liquid culture filtrates were measured. The results showed that rice straw was the best carbon source, and urea was the best nitrogen source for 14 - 7 and 15 - 1. Optimal culture temperature of ligninase production was 30 ℃. In the optimal culture condition, the lignin peroxidase activity and manganese peroxidase activity of strain 14 -7 were 0. 350 U ·mL^-1 and 0. 080 U ·mL^-1 , respectively. The strain 15 - 1 was able to accumulate an average of 0. 300 U ·mL^-1 and 0. 072 U ·mL^-1 , respectively.

关 键 词：木质素降解菌 木质素酶 优化 木素过氧化物酶 锰过氧化物酶 漆酶 

分 类 号：Q939.9[生物学—微生物学]