甜菜BvM14-MADS box基因启动子的组织特异性表达  

Tissue-specific expression of BvM14-MADS box gene promoter

作　　者：赵晨曦[1] 李沛锦[1] 吴川[1] 王宇光[1] 李海英[1,2] 

机构地区：[1]黑龙江大学生命科学学院黑龙江省普通高等学校分子生物学重点实验室,哈尔滨150080 [2]黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150500

出　　处：《黑龙江大学自然科学学报》2011年第6期848-852,共5页Journal of Natural Science of Heilongjiang University

基　　金：国家自然科学基金资助项目(3087156631071473);黑龙江省教育厅科学技术研究项目(11541284);黑龙江大学高层次人才创新团队支持计划(Hdtd2010-05);黑龙江大学学生科技创新项目

摘　　要：MADS-box基因在植物的花器官和各种营养器官中均有不同形式的时空表达模式,行使不同功能。实验室前期获得了甜菜M14品系BvM14-MADS box基因的特异启动子Pbm,并对其进行了瞬时表达。本试验利用含有重组载体pBI121-Pbm-GUS的农杆菌转化烟草,对报告基因GUS在转基因烟草各组织表达特性进行研究,探讨BvM14-MADS box基因特异启动子Pbm是否具有组织特异性表达活性。试验结果表明,GUS基因只在花的萼片中有表达,在根、茎、叶中均无表达,可见此启动子Pbm在花组织的萼片中具有表达特异性。The members of MADS-box family have different spatial and temporal expression patterns in flower meristem, floral organs and a variety of vegetative organs, and play different functions. In previous study, the BvM14- MADS box promoter Pbm was cloned and made it transient expression, but didn＇ t verified its expression pattern in different tissues of the transgenic tobacco. The tobacco with the engineeed Agrobacterium tumefaciens EHA105 was transformed that contained the recombinant plasmid pBI121-Pbm-GUS with the promoter Pbm replaced the 35S promoter. Followed selecting the positive plants, the expression of GUS in different tissues of transgenic tobacco was tested. The result showed that the GUS gene only can be expressed in sepal, but not in leaf, stem and root, indicating that the BvM14-MADS box promoter Pbm had a special activity in floral organ.

关 键 词：BvM14-MADS BOX基因 启动子 遗传转化 组织特异性表达 

分 类 号：S566.3[农业科学—作物学]